磁性分子印迹电化学传感法检测痕量奥卡西平

Detection of Oxcarbazepine Using an Electrochemical Sensor Based on Magnetic Molecularly Imprinted Membrane

该文以4-乙烯基吡啶和甲基丙烯酸酯为原料制备了一种可用于检测奥卡西平(OXC)的磁性分子印迹电化学传感器(MNPs-MIP/MCPE)。首先,依据密度泛函数理论(DFT/B3LYP/6-31+G)计算,实验成功地筛选和构建出OXC与功能单体的最佳组合及比例。随后,基于沉淀聚合法合成了能够识别OXC的磁性分子印迹膜(MNPs-MIP),将MNPs-MIP覆于碳糊电极(MCPE)表面制成MNPs-MIP/MCPE。采用差分脉冲伏安法(DPV)将MNPs-MIP/MCPE传感器用于不同浓度OXC的测定。结果显示,传感器的峰电流信号随OXC浓度的增大而增大,且OXC分别在5×10^(-8)^3×10^(-6)mol/L和3×10^(-6)^1.5×10^(-4)mol/L浓度范围内与其峰电流信号呈线性关系,其线性方程分别为:I_(p)(μA)=1.755+1.097c(μmol/L),相关系数(r)=0.9997和I_(p)(μA)=0.131+5.177c(μmol/L),r=0.9996。OXC的检出限(LOD=3S/m)为2.06×10^(-8)mol/L。该传感器成功用于实际样品中OXC含量的检测,其回收率为99.4%^101%,相对标准偏差(RSD)为1.5%^2.5%。

In this paper,a magnetic molecularly imprinted electrochemical sensor(MNPs-MIP/MCPE)for the detection of oxcarbazepine(OXC)was prepared,using 4-vinylpyridine and methacrylate as raw materials.Firstly,according to the calculation of the density functional theory(DFT/B3LYP/6-31+G),the best combination and the best combination ratio of the OXC with functional monomers were successfully screened and constructed.Subsequently,a magnetic molecularly imprinted membrane(MNPs-MIP)capable of recognizing OXC was synthesized based on the precipitation polymerization method,which was coated on the surface of a carbon paste electrode(MCPE)to make a magnetic molecularly imprinted sensor of MNPs-MIP/MCPE.The sensor was used for the determination of different concentrations of OXC solutions with differential pulse voltammetry(DPV).Results showed that,the current signals of the system had good linear relationship(r>0.999)with OXC concentrations in the ranges of 5×10^(-8)-3×10^(-6) mol/L and 3×10^(-6)-1.5×10^(-4) mol/L,and their linear equations were I_(p)(μA)=1.755+1.097c(μmol/L)and I_(p)(μA)=0.131+5.177c(μmol/L),respectively.The detection limit for OXC(LOD=3S/m)was 2.06×10^(-8) mol/L.The sensor was used for the detection of OXC concentration in the actual samples,with recoveries of 99.4%-101%and relative standard deviations(RSD)of 1.5%-2.5%.