参虎败毒颗粒体内抗猪繁殖与呼吸综合征病毒的效果观察

Observation of Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Shenhu Baidu Granules in vivo

为探究参虎败毒颗粒抗猪繁殖与呼吸综合征病毒(PRRSV)的作用,将20头PRRSV阴性仔猪随机平均分为4组,即提前药物处理感染组(A组)、感染同时药物处理组(B组)、无药物处理感染组(C组)和无药物处理不感染组(D组)。A和B组分别于攻毒前3 d和攻毒时在基础日粮中添加参虎败毒颗粒(每头10 g·d-1),连续饲喂至攻毒后28 d,每日测量体温,在攻毒后第14天,每组随机处死仔猪1头,观察病理变化,RT-qPCR检测肺组织病毒载量,并在攻毒后第1、3、5、7、14、21、28、35天采全血和血清样品进行PRRSV核酸和抗体检测。结果发现,A和B组仔猪体温在攻毒后第6天上升至40℃以上,第12天时恢复正常;C组仔猪体温在第5天上升至40℃以上,持续至试验结束仍未恢复。A、B组仔猪肺组织病变较轻,肺泡结构轻度损伤,支气管内有少量炎性渗出物,周围有少量炎性细胞浸润;C组仔猪肺组织病变严重,肺泡结构破坏严重,支气管内有大量炎性渗出物和炎性细胞浸润。A组在攻毒后3、5、7和14 d病毒载量极显著低于C组(P<0.01);B组在攻毒后3和5 d病毒载量极显著低于C组(P<0.01),第7和14天病毒载量显著低于C组(P<0.05)。A、B组PRRSV抗体在攻毒后第21天达到峰值,C组在28 d达到峰值。以上结果可知,参虎败毒颗粒能够显著减轻PRRSV造成的仔猪肺部病理损伤,降低病毒血症;添加药物可使PRRSV特异性抗体峰值提前,减少病毒血症持续时间,证明参虎败毒颗粒可用于预防或治疗PRRSV感染,降低PRRSV对仔猪的影响。

In order to explore the effect of the Shenhu Baidu granules against porcine reproductive and respiratory syndrome virus(PRRSV),20 piglets free of PRRSV were randomly divided into 4 groups.Group A were given medicine at 3 days before the infection and Group B were given medicine and infection at the same time.Group C and D were the infection and non-infection control,respectively.Medicine were mixed in the basal diet(10 g per pig per day)of A,B groups and supplied from the start time points to the end of the experiment.The rectal temperature of each piglet was recorded everyday.On the 14th day post infection(PI),one piglet in each group was randomly selected and euthanized to observe the lesions caused by viral infection.The viral loads in lungs were detected with RT-qPCR.And on day 1,3,5,7,14,21,28,and 35 PI,anticoagulant and non anticoagulant blood samples were collected for detection viral loads and antibodies,respectively.The rectal temperature of the piglets in Group A and B were raised up to 40℃at 6th PI and returned to normal at the 11th and 12th days PI.While,high rectal temperature(more than 40℃)of the piglets in Group C lasted to the end of the experiment.The lung lesions in piglets from Group A and B were lighter and with less alveolar structure damage,inflammatory cell invasion and less exudate in the bronchus.However,the lesions in lung from Group C was serious.The alveolar structures were missing and the bronchus in the lung were filled with full of exudates.Serious inflammatory cell invasion were also observed around the bronchus.The viremia of the Group A were highly significantly lower than Group C at 3,5,7,and 14 days PI of PRRSV(P<0.01).And Group B was highly significantly lower than Group C at 3,5 days PI(P<0.01),significantly lower at 7 and 14 days PI(P<0.05).Antibodies against PRRSV in Group A and B reached to the peak at 21 days PI,and the antibodies of the Group C reached to the peak at 28 days PI.In summary,the Shenhu Baidu granules significantly reduced the lesions caused by PRRSV and also the viral loads in bloods and lungs.The drug brought forward of the peak value of PRRSV-specific antibody and reduced the duration of viremia.This study proved that the Shenhu Baidu granules is effective in prevention and treating PRRSV infection.