姜黄素乳液的工艺优化及其抗炎活性研究

Process optimization and anti-inflammatory activity of the curcumin emulsion

以姜黄素为原料,通过高压均质工艺获得姜黄素乳液。以乳液粒径为评价指标,优化工艺参数,并测定姜黄素乳液在光照、温度条件下的稳定性,进一步测试姜黄素乳液对体外脂多糖LPS诱导细胞模型及体内TPA诱导动物模型的抗炎活性及其对炎症因子表达的调控作用。结果表明,姜黄素乳液高压均质工艺条件为60 MPa、35℃、均质处理1 h,循环6次,所制备的姜黄素乳液粒径为(200±50)nm,姜黄素含量为5%,姜黄素乳液及其化妆品配方在光照、60℃条件下稳定性良好;姜黄素乳液浓度为1~50μg/mL对LPS诱导的产生的NO有显著的抑制作用,涂抹剂量为0.4 mg/ear的姜黄素乳液对TPA诱导的小鼠耳肿胀抑制率达57%以上,其可能是通过抑制炎症反应过程中的关键性细胞因子IL-6、TNF-α的分泌和释放从而发挥抗炎作用。

An emulsion containing curcumin was obtained by high pressure homogenization.According to the particle size of the emulsion,the technological parameters for preparation were optimized.The stability of the curcumin emulsion under light and at high temperature was determined.Further,the curcumin emulsion was used to control the antiinflammatory activities in lipopolysaccharide LPS induced cell model in vitro and TPA induced animal model,as well as its regulating effect on inflammatory factors.The results show that the optimized condition of high pressure homogenization for preparing the curcumin emulsion is being homogenized at 60 MPa for 1 h at 35℃,and repeated for 6 cycles.The particle size of the curcumin emulsion thus prepared is(200±50)nm and the curcumin content is 5%.The curcumin emulsion and the cosmetic formulas thereof have good stability under light and at 60℃.The curcumin emulsion has a significant inhibitory effect on NO induced by LPS when the concentration of the curcumin emulsion is 1-50μg/mL,and the inhibition rate of the curcumin emulsion with 0.4 mg/ear against TPA induced ear edema in mice is larger than 57%.The mechanism may be through inhibiting the secretion of key cytokines IL-6 and TNF-αin the inflammatory reaction,thus exerting anti-inflammatory effect.