线粒体靶向过氧化氢荧光探针的研究进展

Research Progress in Mitochondrial Targeting Fluorescent Probes for Hydrogen Peroxide

控制线粒体过氧化氢的含量可以为细胞存活、生长、分化和维持发挥有益的作用.然而,线粒体过氧化氢的异常产生可能会破坏生物大分子以及细胞的结构,导致老化、突变甚至癌症的发生.因此,迫切需要能够在活细胞中特别是在线粒体中对过氧化氢的水平变化进行有效监测的手段.为此,开发、设计了多种监测线粒体中过氧化氢水平变化的荧光探针.以靶向基团结构为分类依据,总结了近年来线粒体靶向过氧化氢荧光探针的研究进展,介绍了三苯基膦鎓盐类探针、吡啶阳离子类探针、喹啉阳离子类探针、吲哚阳离子类探针和其他阳离子型探针,总结了这些探针的结构、荧光行为及生物成像等.这些荧光探针将成为疾病诊断和病理研究过程中有力的分子工具.

Controlling the content of mitochondrial hydrogen peroxide can play a beneficial role in cell survival,growth,differentiation and maintenance.However,the abnormal production of mitochondrial hydrogen peroxide may destroy the structure of biological macromolecules and cells,and promote the occurrence of aging,mutation and even cancer.Therefore,there is an urgent need for means to effectively monitor changes in hydrogen peroxide levels in living cells,especially in mitochondria.To this end,a variety of fluorescent probes have been developed and designed to monitor changes in hydrogen peroxide levels in mitochondria.The recent progress in mitochondrial targeting fluorescent probes for hydrogen peroxide based on the structure of targeting groups is summarized,and triphenylphosphonium probes,pyridinium probes,quinolinium probes,indolium probes,and other cationic probes are introduced,including the structure,fluorescence behavior and biological imaging of these probes.These fluorescent probes will become powerful molecular tools in future disease diagnosis and pathological research.