鸡内金多糖对尖吻鲈幼鱼生长、消化、肠道抗氧化能力和血清生化指标的影响

Effects of polysaccharide from Endothelium corneum gigeriae galli on growth,digestive,intestinal antioxidant capacity and serum biochemical indices of Lates calcarifer

该研究探讨了鸡内金多糖(Polysaccharide from Endothelium corneum gigeriae galli, PEGG)对尖吻鲈(Lates calcarifer)幼鱼生长、消化、肠抗氧化能力和血清生化指标的影响,以为PEGG在尖吻鲈健康养殖中的应用提供参考依据。在饲料中添加0 (对照组)、5 (低剂量组)、20 (中剂量组)和80 g·kg^(-1) (高剂量组)的PEGG饲喂体质量(11.85±1.66) g的尖吻鲈幼鱼8周。结果表明,相较于对照组,拌喂PEGG能:1)明显提高尖吻幼鱼终末体质量、增重率和特定生长率,且高剂量组差异显著(P<0.05);2)显著提升胃肠道中消化酶活性(P<0.05);3)改善肠道组织结构,皱襞高度、肌层厚度和皱襞宽度显著增加(P<0.05);4)提升肠道抗氧化能力,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶活性(GSH-Px)、谷胱甘肽(GSH)浓度和总抗氧化能力(TAOC)显著升高,丙二醛(MDA)浓度显著下降(P<0.05);5)改善血清学指标,其中,各处理组胆固醇浓度显著降低,中剂量组谷丙转氨酶(ALT)和谷草转氨酶(AST)活性明显下降(P<0.05)。综上,拌喂PEGG能够显著提升尖吻鲈幼鱼的消化和肠道抗氧化能力、改善血清生化水平,进而促进生长性能。在该实验条件下,PEGG最适添加量为20 g·kg^(-1)。

To investigate the effects of dietary polysaccharide from Endothelium corneum gigeriae galli(PEGG)supplementation on the growth,digestive,intestinal antioxidant capacity and serum biochemical indices of Lates calcarifer and to provide references for the application of PEGG in healthy culture of L.calcarifer,we had fed juvenile L.calcarifer[Body mass of(11.85±1.66)g]by basal diets with PEGG at 0(Control group),5(Low-dose group),20(Medium-dose group)and 80 g·kg^(-1)(High-dose group)for eight weeks.The results show that compared with the control group,PEGG supplementation groups could:1)increase the final body mass,weight gain rate and specific growth rate of juvenile L.calcarifer,and the difference was significant in the high-dose group(P<0.05);2)increase the digestive enzyme activity significantly(P<0.05);3)improve the histological structure of intestine significantly,indicating that PEGG increased the fold height,fold width and muscular thickness significantly(P<0.05);4)enhance the antioxidant capacity significantly.The superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)activity,concentration of glutathione(GSH)and total antioxidant capacity(T-AOC)increased in the PEGG supplementation groups,while malondialdehyde(MDA)concentration decreased significantly(P<0.05);5)improve the serum biochemical indices.Cholesterol in all the treatment groups decreased significantly,while ALT and AST in the medium-dose group decreased significantly(P<0.05).In conclusion,dietary PEGG can improve the digestive capacity,antioxidant capacity and serum biochemical indices of juvenile L.calcarifer,and promote the growth performance.The optimal PEGG supplementation level is 20 g·kg^(-1).