风热清口服液的抗炎镇痛药效作用及其机制研究

Anti-inflammatory And analgesic Effects of Fengreqing Oral liquid and Its Mechanism

目的:探究风热清口服液的抗炎与镇痛作用并初步阐明其作用机制。方法:取小鼠随机分为模型对照组、阳性对照组(醋酸地塞米松3 mg/kg或盐酸曲马多缓释片30 mg/kg灌胃给药)和风热清口服液低、中、高剂量组(分别以5.0125g/kg、10.025g/kg、20.05g/kg灌胃给药),采用小鼠二甲苯耳肿胀模型评价风热清口服液的抗炎作用及机制,采用小鼠醋酸扭体法和热板法共同评价风热清口服液的镇痛作用及机制。结果:与模型对照组比较,风热清口服液低、中剂量对小鼠耳肿胀具有抑制作用(P<0.05),可不同程度减少小鼠耳组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和组胺含量,提高白介素4(interleukin-4,IL-4)的含量(P<0.05或P<0.01)。风热清口服液低剂量能减少醋酸扭体小鼠的扭体次数和小鼠血清中前列腺素E2(prostaglandin E2,PGE 2)含量(P<0.05),且风热清口服液各剂量均能提高扭体小鼠血清中β-内啡肽(β-endorphin,β-EP)的含量(P<0.05)。风热清口服液中、高剂量组小鼠舔足潜伏期延长(P<0.05或P<0.01),血清中β-EP含量显著提高(P<0.01),PGE 2和缓激肽(bradykinin,BK)含量明显减少(P<0.05或P<0.01)。结论:风热清口服液对小鼠具有抗炎作用,其作用机制可能与降低小鼠耳组织中致炎因子TNF-α、组胺和提高抗炎因子IL-4的含量有关,风热清口服液对小鼠具有镇痛作用,其作用机制可能与降低小鼠血清中的PGE 2、BK含量和提高β-EP含量有关。

Objective:To study the anti-inflammatory and analgesic effect of Fengreqing oral liquid.Methods:Mice were randomly divided into model control group(distilled water,ig),positive control group(dexamethasone acetate solution 3 mg/kg or tramadol hydrochloride sustained-release tablets solution 30 mg/kg,ig)and Fengreqing oral liquid low,medium and high dose groups(5.0125g/kg,10.025g/kg,20.05g/kg,ig).The classical model of ear swelling induced by xylene to evaluate the anti-inflammatory effects.The analgesic effect was evaluated by acetic acid writhing model and hot plate model in mice.Results:Compared with the model control group,ear swelling in mice was inhibited by low and medium dose groups of Fengreqing oral liquid(P<0.05).The contents of tumor necrosis factor-α(TNF-α)and histamine in mice ear tissues were reduced,and the content of interleukin-4(IL-4)in mice ear tissues was increased(P<0.05 or P<0.01).The number of writhing times and prostaglandin E2(PGE 2)in the serum of the acetic acid writhing mice were reduced by low dose of Fengreqing oral liquid(P<0.05),andβ-endorphin(β-EP)in the serum of writhing mice were increased by each dose of Fengreqing oral liquid(P<0.05).The latent period of licking paw of mice in the middle and high dose groups of Fengreqing oral liquid was prolonged(P<0.05 or P<0.01).The content ofβ-EP in the serum of mice was significantly increased(P<0.01),The content of PGE 2 and bradykinin(BK)in the serum of mice was reduced(P<0.05 or P<0.01).Conclusion Fengreqing oral liquid has anti-inflammatory effect on mice.Its mechanism may not only be related to reducing the contents of TNF-αand histamine,but also related to increasing the content of IL-4 in mouse ear tissues.Fengreqing oral liquid has analgesic effect on mice.Its mechanism may not only be related to reducing the content of PGE 2 and BK,but also related to increasing the content ofβ-EP in the serum of mice.