黄芪多糖对哮喘小鼠气道炎症启动因子TSLP和DCs表达的影响

Effect of astragalus polysaccharide on the expression of TSLP and DCs in airway inflammation of asthmatic mice

目的研究黄芪多糖(APS)对支气管哮喘模型小鼠气道上皮中胸腺间质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)和树突状细胞(dendritic cell,DCs)表达的影响。方法饲养雌性BALB/c小鼠30只,随机将其分为对照组、哮喘模型组和黄芪多糖组,各组采取相应的干预措施。通过支气管哮喘激发试验、肺组织病理学和酶联免疫吸附试验(ELISA)评价哮喘模型造模是否成功;肺组织中TSLP mRNA的相对表达水平采用实时荧光定量PCR(qRT-PCR)检测;蛋白免疫印迹实验(Western blot)检测肺组织中TSLP蛋白的表达;流式细胞术检测支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中DCs表面CD40、CD80和CD86的表达水平。结果哮喘模型组小鼠气道反应性增高和肺组织HE染色结果均为哮喘的典型表现且哮喘模型组BALF中IL-13、IL-5和IL-4的表达水平显著高于黄芪多糖组和对照组(P<0.001);黄芪多糖组和对照组小鼠肺组织中TSLP mRNA的表达水平与哮喘模型组相比较低(P<0.001),TSLP mRNA的表达水平在黄芪多糖组与对照组之间差异无统计学意义(P>0.05);黄芪多糖组与对照组小鼠肺组织中TSLP蛋白的表达与哮喘模型组相比较弱;黄芪多糖组与对照组BALF中DCs表面CD54、CD80、CD86的表达明显低于哮喘模型组(P<0.001),黄芪多糖组与对照组之间差异无统计学意义(P>0.05)。结论黄芪多糖可明显抑制哮喘模型小鼠气道上皮细胞TSLP水平和DCs表面CD54、CD80、CD86的表达,并减轻气道炎症反应。

Objective To investigate the effects of astragalus polysaccharide on the expression of thymic interstitial lymphopoietin(TSLP)and dendritic cells(DCs)in airway epithelium of bronchial asthma model mice.Method Thirty female BALB/c mice were reared and randomly divided into the astragalus polysaccharide group,the control group,and the asthma model group,and the corresponding intervention measures were taken in each group.The success of asthma modeling was evaluated by the bronchial asthma challenge test,lung histopathology,and the enzyme-linked immunosorbent assay(ELISA).The relative expression level of TSLP mRNA in the lung tissue was detected by real-time fluorescent quantitative PCR(qRT-PCR).Western blot was used to detect TSLP protein expression in the lung tissue.Flow cytometry was used to detect the expression of CD40,CD80,and CD86 on dendritic cells(DCs)surface in bronchoalveolar lavage fluid(BALF).Results The increased airway responsiveness and HE staining results of the lung tissue in mice in the asthma model group are typical of asthma.The expression levels of IL-13,IL-5,and IL-4 in BALF in the astragalus polysaccharide group and the control group were significantly lower than those in the asthma model group (P<0.001). The expression level of TSLP mRNA in the lung tissue of mice in the astragalus polysaccharide group and the control group was lower than that in the asthma model group (P<0.001). There was no significant difference in TSLP mRNA expression between the astragalus polysaccharide group and the control group (P>0.05). The expression of TSLP protein in the lung tissue of mice in the astragalus polysaccharide group and the control group was weaker than that in the asthma model group. The expression of CD54, CD80, and CD86 on the surface of DCs in the astragalus polysaccharide group and the control group was significantly lower than that in the asthma model group (P<0.001), and there was no significant difference between the astragalus polysaccharide group and the control group (P> 0.05). Conclusion Astragalus polysaccharide can significantly reduce the TSLP level of airway epithelial cells and the expression of CD54, CD80, and CD86 on the surface of DCs in asthmatic model mice, and reduce the airway inflammation.