免疫印迹法和ELISA检测过敏原结果比较

Comparison of Allergen Detection Results by Western Blotting and ELISA

目的探讨免疫印迹法和ELISA检测过敏原结果比较。方法选取2018年1月至2019年1月在沈阳金域医学检验所有限公司实验室诊断部的80例样本,对血清样本进行检测,血清样本为免疫印迹法检测过敏原且另外之后用ELISA再检测过敏原结果,比较两组检查方法检验差异。结果两种方法测定的区别在于,家尘和肉制品中的过敏原有一定差异,有统计学意义(P<0.05),其余过敏原无显著差异(P>0.05)。结论免疫印迹法和酶联免疫吸附法各有优缺点,但由于缺乏针对特异性IgE抗体和过敏原国际标准,不同方法获得的结果不一定相同,故应注意不同方法差异及相关性。

Objective To compare the results of immunoblot and ELISA in the detection of allergens.Methods A total of 80 samples from the laboratory diagnostic department of shenyang jinyu medical laboratory co.,ltd.from January 2018 to January 2019 were selected for the detection of serum samples.The serum samples were used for the detection of allergens by western blot and the results of allergen detection by ELISA later.Results The difference between the two methods was that there was a certain difference in allergies between domestic dust and meat products,which was statistically significant(P<0.05),while there was no significant difference in other allergens(P>0.05).Conclusion Western blot and ELISA have their own advantages and disadvantages.However,due to the lack of international standards for specific IgE antibodies and allergens,the results obtained by different methods may not be the same.Therefore,attention should be paid to the differences and correlation between different methods.