基于绿色生物模板电极的微囊藻毒素-LR快速检测

Rapid Detection of Microcystin-LR Based on Green Biotemplated Electrode

为实现水环境中微囊藻毒素的高性能检测,利用农业废弃物合成了具导电性能的生物炭颗粒,并将其作为导电材料构建了对微囊藻毒素-LR具电化学免疫检测性能的生物模板电极。实验结果表明:该电极具有很好的电催化性能,在电解液中,能够在短时间(5 min)内有效识别微囊藻毒素-LR的存在;利用差分脉冲伏安法检测发现在0.0~5.0 nmol/L的检测范围内毒素浓度与响应电流值呈线性递减关系,且最低检测浓度为0.2 nmol/L。该电极对各类不相关分子及离子具有抗干扰能力,表明了该方法可应用于对微囊藻毒素-LR污染的快速、特异性检测。

For the effective detection of microcystin-LR(MCLR) in environmental water, in this study, the biochar particles were prepared from agricultural wastes, and it was utilized for the construction of a biotemplated electrode with electrochemical immunoassay capability toward microcystin-LR by differential pulse voltammetry. It is found that the electrode can identify the presence of microcystin-LR effectively in a short time(5 min), the linear detection range is 0.0-5.0 nmol/L and the lowest detection limit is 0.2 nmol/L. The electrode is also anti-interfere to various unrelated molecules and ions, indicating that the electrode can be used for fast and effective detection of microcystin-LR.