巴西苏木素对肝癌细胞增殖能力的影响

Effect of Brazilin on the proliferation of hepatocellular carcinoma cells

目的探索巴西苏木素对人肝癌细胞MHCC97H增殖能力的影响。方法把人肝癌细胞MHCC97H分为对照组和实验组。对照组在DMEM培养基中加入与巴西苏木素等体积的无水乙醇进行培养;实验组给予含60μg·mL^(-1)巴西苏木素的DMEM培养基进行培养。用CCK8检测细胞的增殖能力,用蛋白质印迹法检测多肿瘤抑制基因1(P16)和周期蛋白依赖性激酶4/6(CDK4/6)的表达水平。结果实验组和对照组的第5天细胞活力(光密度)分别为1.39±0.08和2.21±0.11,P16蛋白相对表达量分别为1.18±0.21和0.43±0.05,CDK4蛋白相对表达量分别为0.87±0.12和1.46±0.17,CDK6蛋白相对表达量分别为0.90±0.11和1.53±0.21,差异均有统计学意义(均P<0.05)。结论巴西苏木素能通过上调肝癌细胞MHCC97H中P16的表达,降低CDK4/6的表达,从而抑制其增殖能力。

Objective To explore the effect of Brazilin on the proliferation of hepatocellular carcinoma cells MHCC97H.Methods Human liver cancer cells MHCC97H were divided into control and experimental groups.The control group was cultured with anhydrous ethanol of the same volume as Brazilian hematoxylin in DMEM medium.The experimental group was cultured with DMEM medium containing 60μg·mL^(-1) Brazilin.CCK8 was used to detect the proliferation ability of cells.The expression levels of multiple tumor suppressor genes 1(P16)and cyclin-dependent kinase 4/6(CDK4/6)were detected by Western Blotting.Results In the experimental group and control group,the cell viability(optical density)on the 5th daywere 1.39±0.08 and 2.21±0.11,the relative expression levels of P16 protein were 1.18±0.21 and 0.43±0.05,the relative expression levels of CDK4 protein were 0.87±0.12 and 1.46±0.17,the relative expression levels of CDK6 protein were 0.90±0.11 and 1.53±0.21,respectively.The differences were statistically significant between two groups(all P<0.05).Conclusion Brazilin can inhibit the proliferation of hepatocellular carcinoma cells MHCC97H by up-regulating the expression of P16 and reducing the expression of CDK4/6.