双标线性校正技术用于天麻多成分色谱峰的定性分析

Identification of Gastrodiae Rhizoma chromatographic peaks identi‐fication by liner calibration with two reference substances assisted

建立天麻药材中6种成分的高效液相色谱方法,并采用双标线性校正法计算保留时间,考察预测保留时间的准确性和色谱柱符合率,用于辅助色谱峰的定性分析。选用24根不同品牌和型号的C18色谱柱,测定天麻中6种成分的保留时间,计算平均值得到标准保留时间(SRT),以峰3巴利森苷E和峰6巴利森苷A两个成分为参照物,使用双标线性校正法(LCTRS)预测保留时间,并在4根其他品牌和型号的C18色谱柱上进行方法的验证。同时以巴利森苷E为参照物,计算校正因子,使用相对保留时间法(RRT)预测保留时间。比较双标线性校正法和相对保留时间法的优劣。结果表明,与相对保留时间法相比,双标线性校正法具有更高预测保留时间准确率和色谱柱符合率,色谱柱的适用范围更广。双标线性校正法具有更好地辅助色谱峰定性作用,具有广阔的应用前景。

This study aimed to establish a method for positioning six chromatographic peaks occurred in HPLC profile of Gastrodiae Rhizoma.The"liner calibration with two reference substances"(LCTRS)method was used to calculate the retention time so as to assist in positioning of chromatographic peaks in terms of the prediction accuracy of retention time and the coincidence rate of chromatographic column.A total of 24 C18 chromatographic columns from different brands and types available were used to determine the retention times of six components in Gastrodiae Rhizoma,then the average retention time of each component was obtained as standard retention time(SRT).Parishin E(peak 3)and Parishin A(peak 6)were simultaneously taken as reference substance to forecast the retention time of the other four components by using the LCTRS method.Four different C18 columns were employed to verify the method.Meanwhile,for the purpose of comparison,the relative retention time(RRT)method was applied to forecast the retention time,by using Parishin E as the single reference substance.The comparison between LCTRS and RRT methods indicated that the former was more accurate in predicting the retention time and more applicable in utilization of chromatographic columns.This study demonstrated that the LCTRS method shows the superior performance in positioning of chromatographic peak,and therefore has a good prospect of application.