枸杞水提液对海洛因损伤小鼠视网膜组织结构、诱导型一氧化氮合酶活性、Caspase-3及核因子κB蛋白表达的影响

Effect of Lycium barbarum soup on histological structure,inducible nitric oxide synthase activity,Caspase-3 and nuclear factor-κB protein expression in retina of mice injured by heroin

目的探讨枸杞水提液对海洛因损伤小鼠视网膜组织结构及相关活性物质的影响。方法120只昆明小鼠随机分为对照组、海洛因组、枸杞1组和枸杞2组。海洛因组和枸杞1组和2组用递增剂量(2、3、4 g/L)腹腔连续(1~5 d、6~10 d和11~15 d)注射海洛因溶液15 d,每天两次(9∶00 am,16∶00 pm),每次0.2 ml,对照组注射等量生理盐水,注射海洛因溶液1 h后,枸杞1组和2组再分别灌胃(1.0 g/kg、2.0 g/kg)枸杞水提液0.2 ml,对照组和海洛因组灌胃等量生理盐水。用生物显微技术观察小鼠视网膜组织结构的变化,用比色法检测小鼠血浆乳酸脱氢酶(LDH)活性和视网膜组织诱导型一氧化氮合酶(i NOS)活性的变化,用免疫组织化学法检测视网膜组织Caspase-3和核因子(NF)-κB蛋白表达的变化。结果与对照组相比,海洛因组小鼠视网膜组织有不同程度的损伤,神经节细胞出现空泡样变性,内核层和外核层结构紊乱,血浆LDH活性显著升高,差异显著(P<0.05或P<0.01),视网膜组织i NOS活性增加,差异极显著(P<0.01);视网膜组织Caspase-3和NF-κB蛋白的阳性表达量升高,平均吸光度值明显增大(P<0.05或P<0.01)。与海洛因组相比,枸杞1组和2组小鼠视网膜组织神经节细胞空泡化减轻,内核层和外核层排列较为整齐,血浆LDH活性显著降低,差异显著或极显著(P<0.05或P<0.01),视网膜组织iNOS活性均又下降,差异显著或极显著(P<0.05或P<0.01);视网膜组织Caspase-3和NF-κB蛋白阳性表达又降低,差异极显著(P<0.01)。结论枸杞水提液可提高细胞的抗氧化能力和抗炎能力,抑制视网膜组织Caspase-3的表达和NF-κB的活化,可有效减轻海洛因对视网膜组织的损伤,对视网膜组织有一定的保护作用。

Objective To explore the effects of Lycium barbarum soup on retina histological structure and related active substances in mice injured by heroin.Method Totally 120 Kunming mice were randomly divided into control group,heroin group,Lycium barbarum 1 group and Lycium barbarum 2 group.The mice were intraperitoneally injected escalation with doses(2,4,6 g/L)heroin 0.2 ml continuous(1-5、6-10 and 11-15 days)for 15 days(twice a day).One hour after injected heroin,Lycium barbarum 1 and 2 group was intragastric administration with(1.0 g/kg,2.0 g/kg)Lycium barbarum soup for 0.2 ml.The changes of retina tissue structure were observed by biomicroscopy,the changes of plasma lactate dehydrogenase(LDH)activity and retina inducible nitric oxide synthase(i NOS)activity were detected by colorimetry,the changes of Caspase-3 and nuclear factor(NF)-κB protein expression in retina was detected by immunohistochemistry method.Result Compared with the control group,the retina tissue of the heroin group was damaged in varying degrees,ganglion cells decreased,vacuolated degeneration occurred,the structure of the inner and outer nuclear layers was disordered,loose and reticular.The LDH activity of plasma increased significantly(P<0.05 or P<0.01).The iNOS activity of retina increased significantly(P<0.01).The Caspase-3 and NF-κB protein expression of retina increased,and the average optical density increased significantly(P<0.05 or P<0.01).Compared with the heroin group,the ganglion cell layer vacuolation of the retina in Lycium barbarum 1 and 2 groups was reduced,the inner and outer nuclear layers were arranged orderly.The LDH activity of plasma decreased significantly(P<0.05 or P<0.01).The i NOS activity of retina decreased significantly(P<0.05 or P<0.01).The Caspase-3 and NF-κB protein expression of retina decreased significantly(P<0.01).Conclusion Lycium barbarum soup can improve the anti-oxidation and anti-inflammatory ability of cells,inhibit Caspase-3 expression and NF-κB activation of retina.It can effectively reduce the damage of heroin on the retina and has a certain protective effect on the retina.