铁钯纳米颗粒修饰的碳纳米管对人乳腺癌MCF-7细胞的放射增敏作用

Radiosensitivity enhancement effect of FePd@CNTs nanocomposites on MCF-7 cells

目的探究铁钯纳米颗粒修饰的碳纳米管(FePd@CNTs)对人乳腺癌MCF-7细胞的放射增敏作用。方法用化学还原法制备FePd@CNTs,并通过透射电子显微镜、能谱仪进行表征。CCK-8法检测FePd@CNTs对人正常乳腺上皮MCF-10A细胞的相容性。CCK-8法、流式细胞术和克隆形成实验评估FePd@CNTs对人乳腺癌MCF-7细胞的放射增敏作用。结果FePd纳米颗粒成功地通过化学还原方法被修饰在CNTs的表面。FePd@CNTs对MCF-10A细胞显示低细胞毒性(IC50=738.3μg/m),同时可有效增强X射线对MCF-7细胞的杀伤(增敏比为1.22)。结论FePd@CNTs具有对乳腺癌的治疗作用与放射增敏的潜力。

Objective To evaluate the radiosensitivity enhancement effect of FePd@CNTs nanocomposites on human breast cancer MCF-7 cells.Methods FePd@CNTs nanocomposites were synthesized by chemical reduction method.Transmission electron microscope and energy dispersive spectrometer were utilized to characterize the surface morphology and chemical composition of FePd@CNTs nanocomposites.The compatibility of FePd@CNTs nanocomposites with human normal breast epithelial MCF-10A cells was determined by CCK-8 assay.The radiosensitivity enhancement effect of FePd@CNTs nanocomposites on MCF-7 cells was assessed by CCK-8 assay,flow cytometry and clony formation assay.Results FePd nanospheres were successfully modified on the surface of CNTs by chemical reduction method.FePd@CNTs nanocomposites showed a low toxicity to MCF-10A cells(IC50=738.3μg/m),and effectively enhanced the effect of X-ray radiation on MCF-7 cells(sensibilization ratio=1.22).Conclusion FePd@CNTs nanocomposites exhibit a promising potential for treating breast cancer and enhancing radiosensitivity effect.