HPLC法测定院内制剂参芪益母膏中芍药苷、芍药内酯苷的含量

Determination of Paeoniflorin and Alibiflorin in ShenQi YiMu Ointment by HPLC

目的建立参芪益母膏中芍药苷、芍药内酯苷的高效液相色谱含量测定方法。方法色谱柱为AgilentC 18(4.6×250 mm,5μm),柱温:25℃;流动相:0.1%磷酸水溶液-乙腈(85∶15),流速1 mL·min^(-1),进样量:10μL,检测波长:230 nm。结果芍药苷、芍药内酯苷在测定范围内(0.0102~0.1224μg,0.0138~0.1656μg)呈良好的线性关系;芍药苷平均加样回收率为98.92%(n=6),芍药内酯苷平均加样回收率为98.87%(n=6),RSD分别为0.23%和0.18%。结论该方法简单、可靠、重复性好,可用于参芪益母膏质量控制。

OBJECTIVE To establish a HPLC method for the determination of paeoniflorin and alibiflorin in ShenQi YiMu ointment.METHODS The column was Agilent C18(4.6×250 mm,5μm),column temperature:25℃,mobile phase:0.1%phosphoric acid aqueous solution acetonitrile(85∶15),flow rate:1 mL·min^(-1),injection volume:10 mL,detection wavelength:230 nm.RESULTS There was a good linear relationship between paeoniflorin and alibiflorin in the determination range(0.0102-0.1224μg,0.0138-0.1656μg);the average recovery of paeoniflorin was 98.92%(n=6),the average recovery of alibiflorin was 98.87%(n=6),RSD was 0.23%and 0.18%,respectively.CONCLUSION The method is simple,reliable and repeatable,and can be used for the quality control of ShenQi YiMu ointment.