丙泊酚调节脂多糖诱导的RAW264.7单核巨噬细胞M1/M2型极化的研究

Propofol Regulates Lipopolysaccharide-induced RAW264.7 Monocyte Macrophage M1/M2 Polarization

目的研究丙泊酚对脂多糖诱导的RAW264.7单核巨噬细胞M1/M2型极化的影响.方法不同剂量的丙泊酚处理脂多糖诱导的RAW264.7单核巨噬细胞,CCK8检测细胞活力,流式细胞术检测细胞凋亡率和线粒体膜电位的变化,Western印迹检测Bax/Bcl-2、iNOS和Arg-1蛋白质水平,RT-PCR检测iNOS、IL-1β、Arg-1和IL-10 mRNA水平,ELISA检测IL-6、TNF-α、IL-4和IL-10含量.结果与模型组比较,25和50μmol/L丙泊酚组细胞活力显著升高(P<0.05),细胞凋亡率和Bax/Bcl-2蛋白质水平显著降低(P<0.05),JC-1红绿荧光比值显著升高(P<0.05),iNOS和IL-1βmRNA水平显著降低,Arg-1和IL-10 mRNA水平显著增高(P<0.05),IL-6和TNF-α含量显著降低,IL-4和IL-10含量显著增高(P<0.05).结论丙泊酚能够抑制脂多糖诱导的RAW264.7单核巨噬细胞M1型极化,促进M2型极化.

Objective To study the effect of propofol on the M1/M2 polarization of RAW264.7 monocyte macrophages induced by lipopolysaccharide.Methods Propofol was used to treat lipopo-lysaccharide-induced RA W264.7 monocyte macrophages,CCK8 was employed to detect cell viabili-ty,flow cytometry was utilized to determine the cell apoptosis and changes in mitochondrial mem-brane potential,Western blotting was applied for the detection of Bax/Bcl-2,iNOS,Arg-1 protein level,RT-PCR was used to detect iNOS,IL-1β,Arg-1 and IL-10 mRNA levels,ELISA was em-ployed to measure the levels of IL-6,TNF-α,IL.4 and IL-10.Results Compared with the model group,the cell viability of the 25μmo/L and 50μmoL/L propofol groups was significantly in-creased(P<0.05),the apoptosis rate and Bax/Bc-2 protein level were significantly reduced(P<0.05).The ratio of red to green fluorescence of JC-1 rose significantly(P<0.05),the content of iNOS and IL-1βwere signifieantly lowered,the contents of Arg-1 and IL-10 were significantly el-evated(P<0.05),the content of IL-6 and TNF-a was significantly decreased,the content of IL4 and IL-10 went up significantly(P<0.05).Conclusion Propofol can inhibit the Ml polarization of RAW264.7 monocyte macrophages induced by lipopolysaccharide and promote the M2 polarization.