黏膜相关恒定T细胞在原发性干燥综合征患者唇腺组织中的表达及临床意义

Mucosal-associated invariant T cells expression in the salivary gland and its clinical significance in primary Sjögren′s syndrome

目的探讨黏膜相关恒定细胞(MAIT细胞)在pSS患者唇腺组织中的表达,并分析其临床意义。方法获取32例pSS患者和27例非SS对照患者的唇腺组织病理切片,采用免疫荧光方法检测MAIT细胞的表达水平,并结合临床资料进行分析。采用t检验、方差分析和Spearman相关分析进行统计处理。结果pSS患者唇腺组织中的平均MAIT细胞数量为[(2.40±0.33)个/腺体],显著高于对照组非SS患者的[(0.79±0.13)个/腺体,t=4.24,P<0.01];有口干(26例)症状的pSS患者,其唇腺中MAIT细胞的水平显著高于无口干症状pSS患者[(2.73±0.38)个/腺体和(0.95±0.15)个/腺体,t=2.24,P=0.03];有猖厥龋齿症状(17例)的pSS患者,其唇腺中MAIT细胞的水平也显著高于无猖厥龋齿症状pSS患者[(3.13±0.54)个/腺体和(1.57±0.20)个/腺体,t=2.57,P=0.02];pSS患者唇腺中MAIT细胞的水平与ESR和ESSDAI评分呈显著正相关[分别为r=0.37,P=0.04和r=0.65,P<0.01];SSA抗体阳性(23例)的pSS患者,其唇腺中MAIT细胞的水平更高[(2.89±0.40)个/腺体和(1.13±0.32)个/腺体,t=2.61,P=0.01];唇腺组织中淋巴细胞灶数多的pSS患者,MAIT细胞的水平也更高[1灶:(1.50±0.49)个/腺体,2灶:(2.29±0.52)个/腺体;≥3灶:(3.66±0.59)个/腺体;F=4.22,P=0.02]。结论MAIT细胞在pSS患者唇腺组织中的表达高于非SS患者,且与口腔症状、病情活动及严重程度、抗体产生都有关联,提示MAIT细胞有可能参与了pSS的局部炎症反应,并在pSS的发病中起到一定作用。

Objective To investigate the expression of Mucosal-associated invariant T(MAIT)cells in the salivary gland of primary Sjögren′s syndrome(pSS)patients,and to analyze its clinical significance.Methods Thirty-two pSS patients and 27 non-pSS controls who had salivary gland biopsies were enrolled.The expression of MAIT cells was detected by immunofluorescence and then analyzed based on clinical data.T test,variance analysis and Spearman correlation analysis were used for statistical analysis.Results The expression of MAIT cells in the salivary gland of pSS patients was significantly higher than in controls[(2.40±0.33)/gland and(0.79±0.13)/gland respectively,t=4.24,P<0.01].In pSS patients with mouth dryness(n=26),MAIT cells in the sali-vary gland were significantly increased compared with those without[(2.73±0.38)/gland vs(0.95±0.15)/gland,t=2.24,P=0.03].In patients with dental caries(n=17),MAIT cells in the salivary gland were also significantly increased[(3.13±0.54)/gland vs(1.57±0.20)/gland,t=2.57,P=0.02].The expression of MAIT cells in the salivary gland of pSS patients was positively correlated with erythrocyte sedimentation rate(ESR)and European League Against Rheumatism Sjögren′s Syndrome Disease Activity Index(ESSDAI)scores(r=0.37,P=0.04 and r=0.65,P<0.01 respectively).In pSS patients with positive SSA antibody(n=23),the amount of MAIT cells was more than controls[(2.89±0.40)/gland vs(1.13±0.32)/gland,t=2.61,P=0.01].The level of MAIT cells in the salivary gland was higher in pSS patients with more lymphocytic foci[one foci:(1.50±0.49)/gland,two foci:(2.29±0.52)/gland,three foci(3.66±0.59)/gland;F=4.22,P=0.02].Conclusion The expression of MAIT cells in the salivary gland of pSS patients is significantly higher than non-pSS controls,and is correlated with oral symptoms,disease activity and the production of autoantibodies.This may suggest that MAIT cells may participate in the local inflammation response and play a role in the pathogenesis of pSS.