长非编码RNA-SNHG4促进胃癌细胞增殖生长并介导对化疗药物顺铂耐药性的研究

Long non coding RNA-SNHG4 promotes cell proliferation and mediates cisplatin resistance in gastric cancer

目的建立长非编码RNA(lncRNA)-SNHG4胃癌稳转细胞系,检测SNHG4对胃癌细胞增殖生长的作用及对顺铂敏感性的影响,检测对比SNHG4在胃癌和癌旁组织中的表达,进行SNHG4的表达水平与胃癌患者病理学特征的相关性分析。方法逆转录-实时荧光定量PCR(RT-qPCR)实验检测细胞和组织中SNHG4的表达水平,MTT和3-D细胞培养实验检测细胞活力和细胞增殖生长能力,评价SNHG4过表达对胃癌细胞增殖生长和顺铂耐药的作用,卡方检验分析SNHG4表达水平与胃癌患者病理学特征的相关性。结果MTT和3-D细胞培养实验表明SNHG4过表达促进胃癌细胞MKN-45和AGS的增殖生长和顺铂耐药(P<0.05);SNHG4在胃癌组织中的表达水平高于癌旁组织;SNHG4在胃癌组织中的高表达与患者的肿瘤体积大小、淋巴结转移和病理学分级具有相关性(P<0.05),与患者的年龄、性别和肿瘤临床分期无相关性。结论SNHG4促进胃癌细胞增殖生长和顺铂耐药,SNHG4在胃癌组织中高表达并与患者临床病理特征相关。

Objective To establish lncRNA-SNHG4 overexpressing gastric cancer cell lines;to examine the role of SNHG4 on cell growth,proliferation and the sensitivity to cisplatin in gastric cancer cells;to compare the expression levels of SNHG4 in gastric cancer and adjacent normal tissues,and to analyze the correlation between SNHG4 expression levels and pathological characteristics in gastric cancer patients.Methods The expression levels of SNHG4 in cell lines and tissues were detected by RT-qPCR;cell viabilities,cell proliferation and growth ability were detected by MTT and 3-D cell culture assay respectively;the correlation between SNHG4 expression levels and pathological characteristics in gastric cancer patients was analyzed by chi square test.Results MTT and 3-D cell culture experiments showed that overexpression of SNHG4 promoted the proliferation and cisplatin resistance in gastric cancer cell lines MKN-45 and AGS(P<0.05);the expression levels of SNHG4 in gastric cancer tissues were significantly higher than that in adjacent normal tissues;the high expression levels of SNHG4 in gastric cancer tissues were significantly correlated with tumor volume,lymph node metastasis and pathological grade in gastric cancer patients(P<0.05),but not with patients′age,gender or clinical stage.Conclusion SNHG4 promotes cell proliferation,growth and cisplatin resistance in gastric cancer cells.The high expression levels of SNHG4 are correlated with pathological characteristics in gastric cancer patients.SNHG4 has important clinical significance in gastric cancer.