SPNS2在肝细胞性肝癌中的表达及其与上皮间质转化的关系

The expression of SPNS2 in hepatocellular carcinoma and its relationship with epithelial-mesenchyme transition

目的探讨鞘氨醇-1-磷酸转运体(SPNS2)在肝细胞肝癌(HCC)中的表达及其与上皮细胞间质转化(EMT)之间的相关性。方法收集10例HCC新鲜临床标本和70例HCC手术标本石蜡切片(包括癌组织和对应癌旁组织)及其临床相关病理资料,检测SPNS2及EMT的两个主要标志物N-钙黏蛋白(N-cadherin)、E-钙黏蛋白(E-cadherin)在癌及癌旁组织中的表达量,统计分析SPNS2与临床病理特征及EMT之间的相关性。结果SPNS2、N-cadherin在癌组织的表达水平高于癌旁组织(P<0.01),而E-cadherin表达低于癌旁组织(P<0.01)。分析得出,SPNS2与N-cadherin呈正相关性,与E-cadherin呈负相关性。SPNS2在HCC的表达与患者年龄、性别、分化程度无相关性,而与TNM分期和血管侵犯密切相关。结论SPNS2在HCC中存在差异性表达,同时可能通过调控EMT的转录因子促进HCC发生。SPNS2与TNM分期和微血管侵犯密切相关,可用于临床预后评估。

Objective To investigate the correlation between the expression of SPNS2 in hepatocellular carcinoma and its epithelial-mesenchymal transition(EMT).Methods 10 fresh clinical specimens of HCC and 70 paraffin sections of HCC surgical specimens(including cancerous tissues and corresponding adjacent tissues)and clinical related pathological data were collected,and the two main markers of SPNS2 and EMT,N-cadherin(N-cadherin),E-cadherin(E-cadherin)expression in cancer and adjacent tissues were detected,and the correlation between SPNS2 and clinicopathological features and EMT was analyzed.Results The expression level of SPNS2,N-cadherin in cancer tissues were higher than those in adjacent tissues(P<0.01),and the expression of E-cadherin was lower than that of adjacent tissues(P<0.01).The analysis showed that SPNS2 was positively correlated with N-cadherin and negatively correlated with E-cadherin.The expression of SPNS2 in HCC was not associated with the patient′s age,gender,and degree of differentiation,but was closely related to TNM grade and microvascular invasion.Conclusion SPNS2 is differentially expressed in HCC,and it may promote the occurrence of HCC by regulating EMT transcription factors.SPNS2 is closely related to TNM grade and microvascular invasion,and can be used for clinical prognosis assessment.