五汁饮对对乙酰氨基酚诱导小鼠肝损伤的保护作用

Protective Effect of Wuzhiyin against Acetaminophen-induced Hepatic Injury in Mice

目的:探讨五汁饮(Wuzhiyin,WZY)对对乙酰氨基酚(acetaminophen,AP)致小鼠急性肝损伤的保护作用。方法:将小鼠随机分为空白组,模型组及五汁饮低、中、高剂量组,灌胃给药100、200、400 mg/kg,空白组和模型组灌胃等体积生理盐水。通过计算脏器指数,检测血清中的ALT、AST及肝组织匀浆中的MDA、GSH、TNF-α、IL-6、IL-1β水平,结合肝组织切片来观察病理学变化。结果:五汁饮低、中、高剂量组小鼠肝脏指数较模型组降低(P<0.05或P<0.01)。与模型组相比,五汁饮中、高剂量明显抑制血清中的AST、ALT活力(P<0.05或P<0.01),显著性降低TNF-α、IL-6、IL-1β的水平(P<0.05或P<0.01),使肝组织匀浆中MDA水平降低,同时缓解肝组织匀浆中GSH水平的升高;组织病理学HE和Tunel染色显示五汁饮可改善肝组织的坏死与凋亡,并减轻细胞炎性浸润。结论:五汁饮对对乙酰氨基酚诱导的急性肝损伤有一定的保护作用,其机制可能与其抗氧化作用、减少炎症反应及抑制细胞凋亡有关。

Objective:To investigate the protective effect of Wuzhiyin(WZY)against acetaminophen(AP)induced hepatic injury in mice.Methods:Male KM mice were randomly divided into blank group,model group,low,medium and high dose WZY groups(100,200,400 mg/kg).The normal saline of equal volume was used in the blank group and model group.In addition to the blank group,the other groups were given AP(300 mg/kg)on the 6th day to establish the model of liver injury in mice.Calculate the organ index to detect alanine aminotransferase(ALT),aspartate aminotransferase(AST)in serum and malondialdehyde(MDA),glutathione(GSH),TNF-α,IL-6,IL-1βlevel,combined with liver tissue sections to observe pathological changes.Results:The liver index of the mice in the low,medium and high dose group were significantly lower than that in the model group(P<0.05 or P<0.01).Compared with the model group,the serum AST,ALT,TNF-α,IL-6,IL-1βin the medium-,high-dose group significantly decreased(P<0.05 or P<0.01).The content of GSH in the medium-,high-dose group significantly increased,while the content of MDA significantly decreased.Histopathological HE and Tunel staining showed that WZY could improve the necrosis and apoptosis of liver tissue,and reduce the inflammatory infiltration of cells.Conclusion:WZY had certain protective effect on acetaminophen-induced acute liver injury,and its mechanism might be related to its antioxidant effect,reduction of inflammation and inhibition of cell apoptosis.