基于多电极的混合溶液中糖组分检测系统设计及应用研究

Study on Design and Application of Sugar Components from Mixed Solution Detection System

糖类是人体必需的能量物质,但过量摄入糖也容易导致多种疾病.所以许多研究人员对某一种糖的定量检测探究了多种不同的方法.然而在自然状态下,糖类并不是单一存在的.本文提出了一种可以在混合糖类溶液中测定各糖类浓度的电化学传感器.本文采用传统的三电极体系,采用Ag/AgCl(饱和KCl)电极作为参比电极,铂片电极作为对电极,工作电极分别采用泡沫镍电极、铜膜电极和铜片电极三种电极.采用伏安循环法(CV)和电流时间法(i-t)检测了混合溶液的电化学响应,混合溶液含不同浓度和比例的葡萄糖、果糖和蔗糖,并采用梯度提升树(GBDT)通过对检测数据进行分析和建模来实现定量检测.结果证明本传感器具有较好的检测效果,检测范围为0.25 mmol/mL~4.76 mmol/mL,平均误差为4.49%.本方法具有响应速度快、检测方便、灵敏度高等优点.为混合溶液中糖类含量的检测提供了一种新的思路.

Sugar is an essential energy substance for human body,but excessive intake of sugar can also lead to a variety of diseases.So many researchers had explored different ways to quantify a sugar.However,in natural states,sugars are always present not alone.In this paper,an electrochemical sensor which can determine the concentration of various sugars in mixed sugar solution is presented.The system adopts the conventional three-electrode system.Ni foam electrode,Cu film electrode and Cu sheet electrode were used as working electrodes.Pt film electrode and Ag/AgCl electrode were used as counter and reference electrodes,respectively.Cyclic Voltammetry(CV)and chrono-amperometry(i-t)was utilized as testing methods.The electrochemical response of mixed sugar solution at different concentrations was detected by the sensor.Mixed solutions contain glucose,fructose and sucrose in different concentrations and ratios.And the Gradient Boosting Decision Tree(GBDT)is adopted to realize quantitative detection by analyzing and modeling the detection data.The results show that the sensor has a good detection effect,the average error is 4.49%,the detection range is from 0.25 mmol/mL to 4.76 mmol/mL.Moreover,the sensor has the advantages of rapid responses,easy operation,high sensitivity,etc.It provided a new way for sugar content analysis from mixed solution.