ISSR技术应用于香蕉种质资源的研究进展

Research Progress of ISSR Technology Applied to Banana Germplasm Resources

香蕉是热带与亚热带地区重要的经济水果,也是我国的经济作物之一。简单序列间重复序列(ISSR)为一种新型的分子标记技术,因其具有模板DNA的用量少、操作简单、快速等优点。常用于植物遗传多样性、种质资源的丰富度等的研究。通过分析ISSR技术的原理与特点,利用其对不同地域、不同国家的多个香蕉品种的亲缘关系与抗性相关方面的研究近况。探讨目前使用ISSR技术存在的问题,并展望其未来的研究前景。

Banana is an important economic fruit in tropical and subtropical regions,and also one of the economic crops in China.Simple intersequence repeat sequence(ISSR)is a novel molecular marker technology,which has the advantages of less template DNA,simple operation and fast operation.Therefore,it is often used in the study of plant genetic diversity and germplasm richness.In this paper,the principle and characteristics of ISSR technology were introduced,as well as the recent research progress on the relationship and resistance of several banana varieties in different regions and countries by using ISSR technology.At the same time,the existing problems of using ISSR technology are discussed,and the future research prospects are in sight.