基于高通量测序的雪茄烟微生物群落结构和多样性分析

Structure and diversity analysis of microbial communities in cigar products by high-throughput sequencing technology

为探究雪茄烟产品细菌和真菌群落的结构和多样性,采用高通量测序技术对10个雪茄烟样品中细菌16S rRNA基因和真菌ITS基因进行测序检测,根据测序数据进行微生物物种丰度分析、α多样性分析、菌群结构分析和聚类分析。结果表明:①所分析的雪茄烟样品中细菌和真菌的多样性丰富,共检出360个细菌属和49个真菌属,其中细菌和真菌群落多样性最高的样品分别是HNL9和WG。②所分析的雪茄烟样品的优势菌门为厚壁菌门和子囊菌门,优势菌属为葡萄球菌属、不动杆菌属、假单胞菌属和曲霉属。③不同样品中细菌和真菌群落多样性和结构存在显著差异,聚类分析显示来自同一产地国的GXB和MT样品菌群分布比较相似。

To explore the structure and diversity of bacterial and fungal communities in cigar products,high-throughput sequencing technology was used to perform the gene sequencing of bacterial 16S rRNA and fungal ITS regions in 10 representative cigar samples.The abundance of sequences,alpha diversity,structure of microbial communities and species clustering were analyzed based on the sequencing data.The results showed that:1)The diversities of bacteria and fungi in the cigar samples were abundant.A total of 360 bacterial genera and 49 fungal genera were identified,and the highest diversities of bacterial and fungal communities were observed in samples HNL9 and WG respectively.2)Firmicutes and Ascomycota were the dominant phyla,and Staphylococcus,Acinetobacter,Pseudomonas and Aspergillus were the dominant genera in the cigar samples.3)Significant differences were observed in the diversities and structures of bacterial and fungal communities among the samples.Cluster analysis indicated that the distributions of microbial communities in samples GXB and MT from the same country were similar.