胰蛋白酶制备乌贼缠卵腺抗氧化活性多肽的研究

Study on Preparation of Antioxidant Activity Polypeptide from Cuttlefish Ovary Gland by Trypsin

[目的]探讨胰蛋白酶制备乌贼缠卵腺抗氧化活性多肽的工艺及体外抗氧化活性。[方法]以乌贼缠卵腺为原料,用胰蛋白酶进行酶解,采用单因素和正交试验优化乌贼缠卵腺抗氧化活性肽的提取工艺;将酶解液超滤分成4个分子段,通过体外检测DPPH清除率、·OH清除率和还原力评价其抗氧化活性。[结果]胰蛋白酶酶解乌贼缠卵腺在加酶量2000 U/g、pH为8.5、料液比1∶3、酶解时间8 h条件下得到的酶解物对DPPH的清除率最大。酶解液经超滤得到的<3 kD分子段的多肽具有良好的DPPH清除能力、·OH清除能力和还原能力。[结论]胰蛋白酶酶解制备的乌贼缠卵腺多肽具有较好的体外抗氧化活性,可作为抗氧化肽的理想来源。

[Objective]To explore the conditions for the preparation of antioxidant peptides from the nidamental glands of cuttlefish by trypsin and study the antioxidant activities of the enzymatic hydrolysates in vitro.[Method]The nidamental glands of cuttlefish were used as raw materials and hydrolyzed by trypsin.Single-factor and orthogonal tests were used to optimize the extraction process for anti-oxidant peptides from the nidamental glands of cuttlefish.The enzyme hydrolysate was ultrafiltered into 4 molecular segments,and its antioxidant activity was evaluated by in vitro detection of DPPH clearance rate,·OH clearance rate and reducing power.[Result]The optimized conditions were as follows:volume of enzyme of 2000 U/g,pH of 8.5,solid-liquid ratio of 1∶3 and hydrolysis time of 8 h.Peptides with a Mw of less than 3 kD exhibited appreciable antioxidant activities,including DPPH scavenging,·OH scavenging and reducing activities.[Conclusion]The peptides from the nidamental glands of cuttlefish by trypsin had excellent antioxidant activities in vitro and could be an ideal source of antioxidant peptides.