HBV相关肝癌组织中微生物宏蛋白质组特征分析

Characterization Analysis of Metaproteomic in HBV-Related Hepatocellular Carcinoma

越来越多的证据表明微生物菌群和肝脏疾病有着密切联系,但是HBV相关肝细胞癌(hepatitis B virus(HBV)-related hepatocellular carcinoma,HCC)人群肝脏组织内的微生物菌群种类和数量仍不清楚。本研究对来自165个HCC人群的肝癌组织和对应癌旁组织的质谱数据进行宏蛋白质组学层面的鉴定。结果显示,在肝癌组织中,绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadetes)、黄单胞菌目(Xanthomonadales)、军团菌目(Legionellales)、颤藻目(Oscillatoriales)等微生物菌群数量出现显著上调。通过将鉴定的微生物蛋白质比对到COG(Clusters of Orthologous Groups)数据库来进行注释,本研究还发现了许多在肝癌组织中高表达的微生物功能分类。对比健康人群的肝脏组织的宏蛋白质组鉴定结果,本研究发现在HCC人群的肝脏组织中,变形菌门(Proteobacteria)的含量降低,厚壁菌门(Firmicutes)的含量升高。这项研究可以对肝脏微生物菌群的失调与HCC发生之间的研究提供更多启发。

More and more evidence shows that the microbiome is closely related to liver diseases,but the taxa and the abundance of liver microbiome of patients with hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC)are still not clear.The study performed metaproteomics identification using mass spectrometry data from liver cancer tissue and corresponding adjacent tissue of 165 HCC patients.The results showed that in liver cancer tissue,the number of microorganisms,such as Chloroflexi,Gemmatimonadetes,Xanthomonadales,Legionellales and Oscillatoriales has increased significantly.Using COG(Clusters of Orthologous Groups)database to annotate identified proteins,the study also found many function categories of microorganisms that are highly expressed in liver cancer tissue.Compared with the results of the proteomic identification of liver tissue from healthy people,the study found that the expression level of Proteobacteria decreased while the expression level of Firmicutes increased in liver tissue of the HCC population.The research could provide more inspiration for the association between dysbiosis of microbial flora in liver and the development of HCC.