孕晚期食源性抗生素暴露及健康影响分析

Analysis of food-borne antibiotic exposure in late pregnancy and its health effects

目的:初步分析孕晚期孕妇及其新生儿食源性抗生素暴露情况,并进一步探讨食源性抗生素暴露对新生儿肠道菌群的影响。方法:选择2018年5月至12月于首都医科大学附属北京妇产医院产检的孕晚期孕妇86例,根据分娩后新生儿脐带血样本的抗生素暴露情况,分为对照组(n=43)和抗生素暴露组(n=43)。采用超高效液相色谱-串联质谱(UPLC-MS/MS)和时间分辨荧光免疫层析法(TRFIA)检测孕晚期孕妇血清、脐带血清、胎便抗生素的暴露情况;同时应用16S rRNA二代测序平台对新生儿肠道菌群进行检测分析,并进行组间比较。结果:孕晚期孕妇血清、脐带血清及新生儿胎便呈现食源性抗生素高比例暴露。抗生素暴露组和对照组的新生儿肠道菌群存在差异,差异有统计学意义(P<0.05)。结论:低剂量、持续暴露的食源性抗生素可通过母体间接影响新生儿肠道菌群,进而影响婴幼儿健康。

Objective:To analyze the exposure of food-borne antibiotics to pregnant women and their newborns in the third trimester of pregnancy,and further explore the impact of food-borne antibiotics exposure on the infant intestinal microbiota.Methods:Totally,86 pregnant women had regular obstetric examinations from May 2018 to December 2018 in Beijing Obstetrics and Gynecology Hospital,Capital Medical University were collected,they were divided into control group(n=43)and antibiotic exposure group(n=43)according to the antibiotic exposure in the neonatal cord blood samples.UPLC-MS/MS and TRFIA were used to detect the exposure of antibiotics to serum of pregnant women in the third trimester,umbilical cord serum,and neonatal feces.16S rRNA second-generation sequencing platform was used to detect and analyze the infant intestinal microbiota.Results:The serum of pregnant women,umbilical cord serum,and neonatal feces results showed a high proportion of food-borne antibiotics exposure.There was a significant difference in infant intestinal microbiota between the antibiotic exposure group and the control group(P<0.05).Conclusions:Low-dose,continuous-exposure food-borne antibiotics can indirectly affect the infant intestinal microbiota through the mother and thus affect the health of infants and young children.