黑蒜生姜大枣苹果醋口服液改善大鼠气虚血瘀及微循环障碍的实验研究

Experiment of black garlic-ginger-jujube-apple vinegar oral liquid on rats of Qi deficiency and blood stasis syndrome or microcirculation disturbance

目的:观察黑蒜生姜大枣苹果醋口服液(GZ)对气虚血瘀证模型大鼠及微循环障碍模型大鼠的影响,研究其行气活血功效。方法:1)实验一。SD雄性大鼠60只,随机分成6组,分别为空白对照组、模型对照组,GZ口服液低(8.33 ml/kg)、中(16.6 ml/kg)、高(24.9 ml/kg)剂量组,芪参胶囊阳性药对照组(0.30 g/kg);通过控制饮食及强迫大鼠在凉水(15℃)中游泳3周制备气虚血瘀证模型,按剂量给药30 d。末次给药1 h后,检测大鼠耳郭微循环血流量,全血及血浆黏度,血内皮素(ET)、6-酮-前列腺素(6-keto-PGF1a)、一氧化氮(NO)含量。2)实验二。大鼠分组、给药方式和剂量均同"实验一",各组按剂量给药30 d,末次给药1h后,舌下静脉给药10%高分子右旋糖酐制备大鼠微循环障碍模型,观察大鼠肠系膜微动脉口径、微静脉口径、红细胞流速变化、血小板聚集功能、血液黏度、红细胞变形指数及大鼠胃、肠、肝微循环血流量的变化。结果:GZ口服液可降低气虚血瘀证模型大鼠全血及血浆黏度,增加舒张血管因子NO、6-keto-PGF1a的含量,减少缩血管因子ET的含量,增加耳郭微循环血流量;亦可增加微循环障碍模型大鼠肠系膜微动脉、微静脉口径,加快红细胞流速,增加胃、肠、肝微循环血流量。结论:GZ口服液具有改善气虚血瘀模型大鼠的气虚血瘀症状及微循环障碍模型大鼠的微循环,表明其具有行气活血的功效。

Objective: To investigate the effect of black garlic-ginger-jujuba-apple vinegar oral liquid(GZ) on a rat model of Qi deficiency and blood stasis syndrome or microcirculation disturbance and the Qi-moving and blood-activating function of GZ.Methods: In experiment 1,60 male Sprague-Dawley rats were randomly divided into blank control group, model control group, low-(8.33 ml/kg),middle-(16.6 ml/kg),and high-dose(24.9 ml/kg) GZ groups, and Qishen capsules positive drug group(0.30 g/kg),and a model of Qi deficiency and blood stasis syndrome was established by diet control and forced swimming in cold water(15°C) for 3 weeks, with drug administration at the corresponding dose for 30 days;related indices were measured at 1 hour after the last administration, including blood flow volume of auricular microcirculation, whole blood viscosity and plasma viscosity, and the content of endothelin(ET),6-keto-PGF1 a,and nitric oxide(NO).Experiment 2 had the same grouping method, administration method, and dose as experiment 1,with drug administration at the corresponding dose for 30 days;at 1 hour after the last administration, the rats were given 10% high-molecular-weight dextran via the sublingual vein to establish a model of microcirculation disturbance, and the rats were observed in terms of diameter of mesenteric arterioles and venules, change in red blood cell velocity, platelet aggregation function, blood viscosity, erythrocyte deformation index, and change in blood flow volume of gastric, intestinal, and hepatic microcirculation.Results: In rats with Qi deficiency and blood stasis syndrome, GZ oral liquid reduced whole blood viscosity and plasma viscosity, increased the content of the vasodilation factors NO and 6-keto-PGF1 a,reduced the content of the vasoconstriction factor ET,and increased blood flow volume of auricular microcirculation;in rats with microcirculation disturbance, GZ oral liquid increased the diameter of mesenteric arterioles and venules, accelerated red blood cell velocity, and increased the blood flow volume of gastric, intestinal, and hepatic microcirculation.Conclusion: GZ oral liquid can improve the symptoms of Qi deficiency and blood stasis in rats with Qi deficiency and blood stasis syndrome and improve microcirculation in rats with microcirculation disturbance, suggesting that it has the function of moving Qi and activating blood.