梧州地区新生儿脐带血红细胞相关参数生物参考区间的建立

Establishment of reference interval of related parameters of neonatal umbilical cord red blood cells in Wuzhou area

目的探索建立梧州地区健康新生儿脐带血红细胞相关参数的生物参考区间,为临床诊疗提供依据。方法采用日本Sysmex XT-4000i全自动血细胞分析仪对729例新生儿脐带血作红细胞相关参数进行分析,采用美国海伦娜SPIFE3000电泳仪对上述脐带血作血红蛋白(Hb)进行电泳,选取未出现Hb Bart′s带的624例新生儿脐带血,对Hb、红细胞计数(RBC)、平均血细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、红细胞分布宽度标准差(RDW-SD)、红细胞分布宽度变异系数(RDW-CV)等作正态性检验,剔除离群值最终入选620例新生儿进行统计分析,建立上述参数的生物参考区间。正态分布的Hb、RBC、血细胞比容(HCT)采用(x±1.96s)作为95%生物参考区间,非正态分布的MCV、MCH、MCHC、RDW-SD、RDW-CV以P 2.5~P 97.5作为95%的参考区间。结果梧州地区新生儿脐带血红细胞相关参数生物参考区间如下,RBC:男(3.38~5.42)×10^(12)/L,女(3.43~5.27)×10^(12)/L;Hb:男121.2~180.8 g/L,女124.1~175.9 g/L;HCT:男36.7%~55.5%,女37.8%~54.2%;MCV:男95.1~116.3 fL,女95.8~114.6 fL;MCH:男31.0~37.6 Pg,女31.0~37.7 Pg;MCHC:男308.0~344.0 g/L,女312.0~341.5 g/L;RDW-SD:男53.2%~76.4%,女52.6%~71.0%;RDW-CV:男14.8%~20.0%,女14.8%~18.9%。结论不同地区新生儿脐带血红细胞相关参数生物参考区间存在差异,有必要建立不同地区的生物参考区间。

Objective To explore the establishment of biological reference interval of umbilical cord blood red blood cell parameters of healthy newborns in Wuzhou area,and to provide basis for clinical diagnosis and treatment.Methods The erythrocyte parameters of 729 cases of neonatal umbilical cord blood were analyzed by Sysmex XT-4000i automatic hematology analyzer,and hemoglobin(Hb)electrophoresis was performed by Helena SPIFE3000 electrophoresis instrument.624 cases without Hb Bart′S-band were selected as the research objects.Normality test was used to eliminate the outliers of Hb,red blood cell count(RBC),mean corpuscular volume(MCV),mean corpuscular hemoglobin content(MCH),mean corpuscular hemoglobin concentration(MCHC),standard deviation of red blood cell distribution width(RDW-SD),Coefficient of variation of red blood cell distribution width(RDW-CV)in each group,and 620 cases of neonatal were included for statistical analysis.Statistical analysis was used to establish the biological reference interval of the above parameters.Hb,RBC and HCT of normal distribution were determined by(x±1.96s)as 95%biological reference interval,and P 2.5-P 97.5 as 95%reference interval for non normal distribution of MCV,MCH,MCHC,RDW-SD,RDW-CV.Results The biological reference intervals of related parameters of neonatal umbilical cord red blood cells in Wuzhou area were as follows,RBC:male(3.38-5.42)×10^(12)/L,female(3.43-5.27)×10^(12)/L;Hb:male 121.2-180.8 g/L,female 124.1-175.9 g/L;HCT:male 36.7%-55.5%,female 37.8%-54.2%;MCV:male 95.1-116.3 fL,female 95.8-114.6 fL;MCH:male 31.0-37.6 Pg,female 31.0-37.7 Pg;MCHC:male 308.0-344.0 g/L,female 312.0-341.5 g/L;RDW-SD:male 53.2%-76.4%,female 52.6%-71.0%;RDW-CV:male 14.8%-20.0%,female 14.8%-18.9%.Conclusion There are differences in biological reference intervals for neonatal cord blood red blood cell parameters in different regions,and it is necessary to establish biological reference intervals in different regions.