P-选择素基因-2123C>G-1817T>C与房颤合并血栓的相关性研究

Relationship between P-selectin gene-2123C>G,-1817T>C and atrial fibrillation complicated with thrombosis

目的探讨P-选择素基因-2123C>G、-1817T>C位点的基因多态性与房颤合并血栓的相关性。方法纳入2011年8月-2020年6月门诊就诊及住院患者、健康体检者,分为房颤血栓组(291例),房颤组(534例),正常对照组(981例)。采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法分析P-选择素基因-2123C>G、-1817T>C的多态性,Logistic回归分析基因与疾病之间的关联强度,使用Haploview软件分析基因的单倍体型。结果P-选择素基因-2123C>G、-1817T>C位点存在多态性,房颤患者携带-1817T>C位点隐性模型可能会增加血栓事件发生的风险。P-选择素-2123C>G/-1817T>C位点单倍体型分别为CC、CT、GC、GT。其中CC、GC单体型增加房颤患者血栓事件发生的风险。结论P-选择素基因-2123C>G、-1817T>C位点的基因多态性与房颤合并血栓有一定的相关性。

Objective To explore the relationship between the polymorphism of P-selectin gene-2123C>G,-1817T>C and atrial fibrillation complicated with thrombosis.Methods From August 2011 to June 2020,291 patients with atrial fibrillation thrombosis,534 patients with atrial fibrillation and 981 patients with normal control were selected.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to analyze the polymorphism of P-selectin gene-2123C>G and-1817T>C.Binary Logistic regression model was used to analyze the correlation between the gene model and disease,and Haploview software was used to analyze the haplotype of gene.Results P-selectin gene is polymorphic at-2123C>G and-1817T>C asites,and patients with atrial fibrillation carrying the recessive model of-1817T>C may increase the risk of thrombotic events.The haplotypes of P-selectin-2123C>G and-1817T>C at locus are CC,CT,GC and GT respectively.Among them,CC and GC haplotypes increase the risk of thrombosis in patients with atrial fibrillation.Conclusion The genetic polymorphism of P-selectin gene-2123C>G and-1817T>C is related to atrial fibrillation complicated with thrombosis.