蛋用鹌鹑的VIPR-1基因的多态性与早期生长性状的关联分析

Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail

为分析VIPR-1基因是否能作为鹌鹑早期生长性状的相关分子标记或者候选基因,以中国黄羽鹌鹑、中国白羽鹌鹑、朝鲜鹌鹑3个蛋用鹌鹑品种作为实验对象,采用PCR-RFLP技术和测序技术检测VIPR-1基因的外显子6-7的多态性,并分析该基因多态性与蛋用鹌鹑早期生长性状的关联程度。结果表明,在3个蛋用鹌鹑品种中的VIPR-1基因外显子6-7区域共检测出15个SNP位点,分别为A94G、G97T、C201T、A204G、A233G、C260G、A299T、C354T、A355G、C378T、C407G、A425C、A543G、G585T、A586G。对HpyCH4IV位点(A355G)进行酶切分析,可以检测到3种基因型即AA(285 bp/811 bp)、AG(285 bp/811 bp/1096 bp)和GG(1096 bp)。中国黄羽鹌鹑和中国白羽鹌鹑的AA、AG、GG基因型频率分别为0.227、0.636、0.136和0.087、0.609、0.304。朝鲜鹌鹑AG和GG基因型频率分别为0.235和0.765。1~4周龄时,VIPR-1基因的HpyCH4IV位点与中国黄羽鹌鹑的日增重、体重、胫长、胸宽、胸深、胸骨长、胫围有显著关联性(P<0.05)。HpyCH4IV位点与中国白羽鹌鹑的胫围有显著关联性(P<0.05)。HpyCH4IV位点与朝鲜鹌鹑的体重、胸宽、胸深有显著关联性(P<0.05)。5~7周龄时,VIPR-1基因的HpyCH4IV位点与中国黄羽鹌鹑的体重、胸骨长、胫长、体长、胸宽、胸深、胫围、日增重和相对生长率均有显著的关联性(P<0.05)。HpyCH4IV位点与中国白羽鹌鹑的日增重和相对生长均有显著的关联性(P<0.05)。HpyCH4IV位点与朝鲜鹌鹑的体重有显著的关联性(P<0.05)。综上研究表明,VIPR-1基因外显子6-7的HpyCH4IV位点(A355G)对蛋用鹌鹑的早期生长性状具有一定影响。

To analyze whether VIPR-1 gene can be used as a molecular marker or candidate gene for early growth traits of quail or not,in this study,three egg quails,namely Chinese yellow quail,Chinese white quail and Korean quail,were used as experimental materials.PCR-RFLP and sequencing were used to detect the polymorphism of exon 6-7 of VIPR-1 gene,and the correlation between the polymorphism and the early growth traits of egg quail was analyzed.The results showed that 15 SNP loci were detected in exon 6-7 of VIPR-1 gene in three egg quails,which were A94G,G97T,C201T,A204G,A233G,C260G,A299T,C354T,A355G,C378T,C407G,A425C,A543G,G585T,A586G.Three genotypes,AA(285 bp/811 bp),AG(285 bp/811 bp/1096 bp)and GG(1096 bp),were detected by restriction enzyme analysis of HpyCH4IV(A355G).The genotypic frequencies of AA,AG and GG were 0.227,0.636,0.136,0.087,0.609,0.304,respectively.The frequencies of AG and GG genotypes in Korean quail were 0.235 and 0.765,respectively.At the age of 1-4 weeks,the HpyCH4IV locus of VIPR-1 gene was significantly correlated with the daily gain,body weight,tibial length,chest width,chest depth,sternal length and tibial circumference of Chinese yellow quails(P<0.05).There was a significant correlation between HpyCH4IV locus and tibial circumference of Chinese white quails(P<0.05).There was a significant correlation between HpyCH4IV locus and body weight,chest width and chest depth of Korean quail(P<0.05).At the age of 5-7 weeks,the HpyCH4IV locus of VIPR-1 gene was significantly correlated with the body weight,sternal length,tibial length,body length,chest width,chest depth,tibial circumference,daily gain and relative growth rate of Chinese yellow quails(P<0.05).There was a significant correlation between HpyCH4IV locus and daily gain and relative growth of Chinese white quails(P<0.05).There was a significant correlation between HpyCH4IV locus and body weight of Korean quail(P<0.05).In conclusion,the HpyCH4IV locus(A355G)in exon 6-7 of VIPR-1 gene has some influence on the early growth traits of quail.