摘要
以“红火球”紫薇茎段为外植体进行组培快繁试验,探讨抗褐化措施。结果表明:春季(4月)采集3 cm长腋芽茎段进行预处理后,用75%酒精灭菌10 s,再用0.1%升汞灭菌10 min,其褐化率和污染率最低,培养效果好;最佳抗褐化诱导培养基为1/2 MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+VC 2.0 g/L+L-半胱氨酸1.5 g/L;最佳抗褐化的“一步法”壮苗生根培养基为1/2 MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+VC 2.0 g/L+L-半胱氨酸1.5 g/L。
The stem segments of Lagerstroemia indica“Dynamite”were used as explants for tissue culture and rapid propagation to study the anti-browning measures.The results showed that the stem segments of 3 cm axillary buds collected in spring(April)were pretreated,sterilized with 75%alcohol for 10 s and then with 0.1%mercuric chloride for 10 min,which had the lowestbrowning rate and pollution rate and the good culture effect;the best anti-browning induction medium was 1/2 MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+VC 2.0 g/L+L-cysteine 1.5 g/L;the culture medium for strong seedlings and rooting of the best“one-step”anti-browning method was 1/2 MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+VC 2.0 g/L+L-cysteine 1.5 g/L.
作者
李芳菲
杨利平
刘资华
时群
陈丽文
何贵整
杨琼
LI Fangfei;YANG Liping;LIU Zihua;SHI Qun;CHEN Liwen;HE Guizheng;YANG Qiong(Qinzhou Academy of Forestry, Qinzhou, Guangxi 535099, China;Guangxi State-owned Qinlian Forest Farm, Qinzhou, Guangxi 535000, China)
出处
《林业调查规划》
2021年第4期44-47,152,共5页
Forest Inventory and Planning
基金
广西钦州市科学研究与技术开发计划项目(201616803).
关键词
“红火球”紫薇
组培快繁
抗褐化
培养基
腋芽萌发
Lagerstroemia indica“Dynamite”
tissue culture and rapid propagation
anti-browning
culture medium
axillary bud germination
