CRIP1基因在人卵巢癌中的表达及其临床意义

Expression and clinical significance of CRIP1 gene in human ovarian cancer

目的通过生物信息学分析富含半胱氨酸肠蛋白1(cysteine-rich intestinal protein 1,CRIP1)基因在人卵巢癌中的表达水平及其临床意义。方法Oncomine数据库分析CRIP1基因在不同类型癌症研究中的表达水平以及在卵巢癌不同病理分型中的表达分布;Kaplan-Meier Plotter工具分析CRIP1基因高表达和低表达与卵巢癌患者预后的关系;STRING数据库以及GEPIA数据库分析CRIP1在卵巢癌中的相互作用蛋白。结果CRIP1基因在不同类型肿瘤中表达差异有统计学意义(P<0.05),在卵巢癌组织中高表达;结合临床特征发现CRIP1高表达的卵巢癌患者预后总生存期及无进展生存期明显降低,尤其在接受紫杉醇、多西他赛以及拓扑替康化疗药物后可导致较差的总生存期;构建CRIP1相关蛋白网络图,在卵巢癌中CRIP1与STK4、YAP1呈负相关,与LGALS3、ATOX1呈正相关;Go聚类分析发现CRIP1参与机体多种生物学通路。结论CRIP1基因可成为诊治卵巢癌的重要靶点和预后判断的关键指标。

Objective To analyze the expression level and clinical significance of cysteine-rich intestinal protein 1(CRIP1)gene in ovarian cancer via bioinformatics methods.Methods Oncomine database was used to analyze the expression level of CRIP1 gene in different types of tumors and pathological types of ovarian cancers.Kaplan-Meier Plotter tool was employed to analyze the relationship between high and low expression of CRIP1 gene and prognosis of ovarian cancer patients.STRING database and GEPIA database were retrieved to analyze CRIP1-interacting proteins in ovarian cancer.Results CRIP1 gene was found to be differentially expressed in various types of tumors(P<0.05),but highly expressed in ovarian cancer tissue.Combined with clinical characteristics,overall survival(OS)and progression-free survival of ovarian cancer patients with the high expression of CRIP1 gene were significantly reduced,and the patients with CRIP1 high expression had a poor OS after chemotherapy with Paclitaxel,Docetaxel or Topotecan.The interaction network diagram of CRIP1 was constructed,and CRIP1 was negatively correlated with STK4 and YAP1,while positively correlated with LGALS3 and ATOX1 in ovarian cancer.Moreover,go clustering analysis showed that CRIP1 was involved in various biological pathways.Conclusion CRIP1 gene could be a significant target for the diagnosis and treatment,as well as a key indicator for the prognosis in ovarian cancer patients.