不同品种绿豆中代谢产物的分离鉴定及代谢机制分析

Separation and Identification of Metabolites and Metabolic Mechanism in Two Cultivars of Mung Bean

利用气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)分离鉴定不同种类绿豆(大明绿豆和九鲤湖绿豆)中的代谢产物。以代谢组学研究方法为基础,以绿豆为研究对象,先利用甲醇溶液提取极性代谢组分,再用N,O-双(三甲基硅烷基)三氟乙酰胺进行衍生化处理,通过GC-MS进行代谢产物的分离鉴定。结果表明:分离鉴定共得到2个品种绿豆的总代谢物67种,其中相同代谢产物30种,但含量均有不同。大明绿豆相对于九鲤湖绿豆的差异代谢物有10种,九鲤湖绿豆相较于大明绿豆的差异代谢物有20种,并且九鲤湖绿豆的糖代谢途径、脂肪酸代谢途径、氨基酸代谢途径、三羧酸循环都比大明绿豆代谢活跃;在大明绿豆中,较为优势代谢产物(>7%)为L-正缬氨酸、肌醇2种,在九鲤湖绿豆中,较为优势代谢产物仅为L-正缬氨酸。这种代谢产物差异性受不同品种的基因差异性、代谢途径和机制差异性等因素的综合影响。

Based on metabonomics,the metabolites in two mung bean cultivars(Daming and Jiulihu)were isolated and identified by gas chromatography-mass spectrometry(GC-MS).The polar metabolits were extracted with aqueous methanol solution and then derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide(BSTFA)before being analyzed by GC-MS.The results showed that a total 67 metabolites were identified in the mung bean cultivars,30 of which were found to be common to be both,but at different levels.Ten differential metabolites were identified in Daming mung beans compared with Jiulihu mung beans,and 20 differential metabolites in Jiulihu mung beans compared with Daming mung beans.The metabolic pathways of sugar,fatty acid,amino acid and TCA in Jiulihu mung beans were more active than in Daming mung beans.The dominant metabolites(>7%)in Daming mung beans were L-norvaline and inositol.The dominant metabolites in Jiulihu mung beans was L-norvaline.We speculated that the difference in the metabolite composition between the two cultivars may be related to the difference in genes,metabolic pathways and other factors.