荧光原位杂交检测HER-2基因扩增和免疫组化技术检测C-erbB-2蛋白表达情况的比较

Comparison of Fluorescence in Situ Hybridization Detection of HER-2 Gene Amplification and Immunohistochemical Detection of C-erbB-2 Protein Expression

目的:比较荧光原位杂交检测(FISH)、免疫组化技术(IHC)在乳腺癌HER-2基因及蛋白检测中的差异性与相关性。方法:以98例乳腺癌患者的手术病理切片为检测对象,分别进行FISH、IHC检测,分析两种检测结果。结果:IHC检测结果显示,阳性占53例(54.08%),阴性占45例(45.92%)。FISH检测结果显示,阳性占比29例(29.59%),阴性占69例(70.41%)。以FISH为金标准,IHC检测为-的符合率为42(93.33%),+的符合率5(17.86%),++的符合率11(78.57%),+++的符合率10(90.91%)。结论:IHC初步筛查C-erbB-2蛋白,-、+++符合率较高,在++、+筛查中IHC符合率较低,建议在IHC筛查基础上再做FISH筛查,以明确HER-2基因状态。

Objective:To compare the differences and correlation between fluorescence in situ hybridization(FISH)and immunohistochemistry(IHC)in the detection of HER-2 gene and protein in breast cancer.Methods:Taking the surgical pathological slices of 98 breast cancer patients as the test objects,FISH and IHC were performed respectively,and the test results were analyzed.Results:IHC test results showed that 53 cases(54.08%)were positive and 45 cases(45.92%)were negative.FISH test results showed that 29 cases(29.59%)were positive and 69 cases(70.41%)were negative.With FISH as the gold standard,the coincidence rate of negative(-)for IHC is 42(93.33%),the coincidence rate of positive(+)is 5(17.86%),the coincidence rate of++is 11(78.57%),and the coincidence rate of+++is 10(90.91)%.Conclusion:In IHC preliminary screening of C-erbB-2 protein,the coincidence rate of-,+++is relatively high,and the coincidence rate of IHC in the screening of++and+is low.It is recommended to do FISH screening on the basis of IHC screening to clarify the HER-2 gene status.