茯苓多糖对小鼠肠道菌群的影响

Effect of Poria cocos Polysaccharide on Intestinal Flora in Mice

目的:基于16S rDNA微生物分类测序分析,通过检测免疫功能受茯苓多糖灌胃影响的小鼠粪便标本,探究茯苓多糖对免疫功能的调节是否与小鼠肠道菌群相关。方法:以水提醇沉法提取茯苓多糖;选择4周龄SPF级雌性小鼠,并随机分为实验组和对照组;分别以茯苓多糖水溶液和生理盐水灌胃14 d;小鼠称重并无菌采集粪便标本;处死小鼠并采集脾脏和胸腺,称重记录;制作胸腺组织切片,荧光染色法计数凋亡细胞数,并统计分析;选取免疫功能受影响的小鼠和对照组小鼠粪便标本,进行粪便细菌微生物分类测序分析,比较两组小鼠肠道菌群的差异。结果:实验组小鼠与对照组小鼠胸腺指数统计p=0.02,脾脏指数统计p=0.93,体重统计p=0.03,胸腺凋亡细胞数统计p=0.001,菌群间差异均p>0.05。结论:茯苓多糖会显著影响小鼠胸腺细胞的发育过程,但并未发现茯苓多糖对小鼠免疫系统的影响与肠道菌群相关的证据。

Objective:Based on 16S rDNA microbial classification and sequencing analysis,the effect of Poria cocos polysaccharide on intestinal microflora of mice was explored by detecting fecal samples of mice whose immune function was affected by Poria cocos polysaccharide.Methods:The polysaccharide of Poria cocos was extracted by water extraction and alcohol precipitation;SPF grade female mice of four weeks old were randomly divided into experimental group and control group;The rats were given Poria cocos polysaccharide solution and normal saline for 14 days;Mice were weighed and collected fecal samples aseptic;The mice were killed and spleen and thymus were collected and recorded;The number of apoptotic cells was counted by fluorescence staining and analyzed;The fecal samples of mice affected by immune function and control group were selected and sent for the analysis of fecal bacteria microbial classification and sequencing,and the differences between the two groups were compared.Results:The thymus index of mice in experimental group and control group was p=0.02,spleen index p=0.93,body weight p=0.03,number of apoptosis cells p=0.001,and the difference of bacteria group p>0.05.Conclusion:Poria cocos polysaccharide can affect the development of thymocytes,but it has no significant effect on intestinal flora.