高危神经母细胞瘤差异表达基因的生信分析

Bioinformatic analysis of differential expression genes in high-risk neuroblastoma

目的应用生信分析方法筛选高危神经母细胞瘤的差异表达基因(DEG)。方法从GEO数据库下载2个高危神经母细胞瘤数据集(GSE49710、GSE73517),筛选DEG,应用GO和KEGG进行富集分析,构建PPI网络筛选中枢基因。结果GSE49710包括34个上调DEG和284个下调DEG,GSE73517包括62个上调DEG和309个下调DEG。GO分析显示,生物过程主要集中在细胞粘附、GTP酶活性的正调节、转录的负调节、DNA模板化、凋亡过程的负调节、中枢神经系统发育,调节的细胞成分富集在膜的组成部分、细胞外区域、质膜的组成部分、细胞外空间,调节的分子功能富集于钙离子结合、受体结合。KEGG分析显示在可卡因成瘾、造血细胞谱系、NOD样受体信号通路、糖胺聚糖生物合成-硫酸乙酰肝素/肝素中显著富集。PPI网络确定5个中枢基因(ADRB2、MC4R、CD69、RBFOX1和IL7R)。结论ADRB2、MC4R、CD69、RBFOX1和IL7R可能与高危神经母细胞瘤的发生、发展相关,也可为高危神经母细胞瘤提供潜在治疗靶点。

Objective To analyze the differential expression genes(DEG)of high-risk neuroblastoma using biosynthesis analysis methods.Methods Two high-risk neuroblastoma datasets(GSE49710,GSE73517)were downloaded from GEO database,and DEG was screened.GO and KEGG analyses were used for enrichment analysis,and a PPI network was constructed to screen central hub genes.Results GSE49710 included 34 up-regulated DEG and 284 down-regulated DEG,GSE73517 included 62 up-regulated DEG and 309 down-regulated DEG.GO analysis showed that biological processes were mainly involved in cell adhesion,positive regulation of GTPase activity,negative regulation of transcription,DNA templating,negative regulation of apoptotic processes,development of the central nervous system,cell components were mainly involved in membranes component,extracellular area,component of plasma membrane and extracellular space,molecular functions were mainly enriched in calcium ion binding and receptor binding.KEGG analysis showed significant enrichment in cocaine addiction,hematopoietic cell lineage,NOD-like receptor signaling pathway,glycosaminoglycan biosynthesis-heparan sulfate/heparin.The PPI network identified five central hub genes,inlcuding ADRB2,MC4R,CD69,RBFOX1 and IL7R.Conclusions ADRB2,MC4R,CD69,RBFOX1 and IL7R may be related to the occurrence and development of high-risk neuroblastoma,and they may also provide potential therapeutic targets for high-risk neuroblastoma.