BEX1和BEX4在口腔鳞状细胞癌中的表达与临床意义

Expression and clinical significance of BEX1 and BEX4 in oral squamous cell carcinoma

目的分析脑表达的X连锁基因1(BEX1)及脑表达的X连锁基因4(BEX4)在口腔鳞状细胞癌(OSCC)组织中及癌旁非肿瘤组织中的表达并探讨其与口腔鳞状细胞癌发生发展的关系。方法使用免疫组化法检测BEX1和BEX4蛋白在OSCC组织及癌旁非肿瘤组织中的表达。TCGA数据库中Kaplan-Meier生存分析BEX1和BEX4的表达与口腔鳞癌患者总生存期的相关性。从String-DB数据库中分析BEX1和BEX4在信号转导中的位置以及相关密切的上下游基因。结果55例OSCC组织中,BEX1蛋白阳性25例(45.45%),BEX4蛋白阳性19例(34.55%);30例癌旁非肿瘤组织中,BEX1阳性24例(80.00%),BEX4阳性27例(90.00%),OSCC组织与癌旁非肿瘤组织相比差异均有统计学意义(P<0.01),BEX1和BEX4蛋白的表达与口腔鳞癌分期、淋巴结的转移与分化程度存在一定的关联,同时其中存在的差异有统计学的意义(P<0.001);然而BEX1与BEX4蛋白的表达与患者的年龄、肿瘤发生部位与性别不存在相关性,差异无统计学意义。关联性分析表明BEX1蛋白和BEX4蛋白表达关联强度为0.489(P<0.001)。Kaplan-Meier生存分析显示BEX1低表达的OSCC患者预后较差。结论BEX1和BEX4可能成为预测OSCC转移的潜在生物学标志物。

Objective To analyze the expressionof brain-expressed X-linked protein1(BEX1)and brain-expressed X-linked protein 4(BEX4)in oral squamous cell carcinoma(OSCC)and adjacent tissues,and to explore its relationship with the occurrence and development of OSCC.Methods The expression of BEX1 and BEX4 proteins in OSCC tissues and non-tumor tissues was analyzed by immunohistochemistry method.Kaplan-Meier survival analysis in TCGA was performed to analyze the correlation between BEX1 and BEX4 expression in OSCC and the overall survival.The location of BEX1 and BEX4 in signal transduction and its upstream and downstream genes related to it were analyzed by the String-DB database.Results Among 55 cases of OSCC,25 cases were positive for BEX1 protein(45.45%),19 cases were positive for BEX4 protein(34.55%),24 cases were positive for BEX1 in non-tumor tissues adjacent to cancer(80.00%),27 cases were positive for BEX4 in non-tumor tissues adjacent to cancer(90.00%).The differences were statistically significant compared with adjacent tissues(P<0.01).The expression of BEX1 protein and BEX4 protein was related with the stage of oral squamous cell carcinoma,lymph node metastasis and differentiation degree,and the difference was statistically significant(P<0.001);but neither of them was related to gender,age and tumor site.The correlation analysis showed that the correlation intensity of BEX1 protein and BEX4 protein expression was 0.489(P<0.001).Kaplan-Meier survival analysis showed that OSCC patients with low expression of BEX1 had a poor prognosis.Conclusion BEX1 and BEX4 may serve as biomarkers for predicting metastasis of OSCC.