核桃青皮中多酚类物质的分离纯化及其抗氧化性

Separation and Purification of Polyphenols from Walnut and Its Antioxidant Activity

试验从核桃青皮中提取纯度较高的多酚类物质,并测定其抗氧化性。以大孔树脂的淋洗吸附作为粗提取方式进行第1次提取,通过调节pH、离心沉淀、降温分离结晶物3个步骤进一步纯化青皮多酚类物质的粗提取液。利用高效液相色谱对比分析粗提取液和纯化液中的青皮多酚,纯化后的多酚类物质含量总和超过80%。试验对影响大孔树脂吸附性能的淋洗流速和影响离心沉淀过程的pH进行探讨分析,并对青皮多酚的抗氧化性方面进行重点分析。

The high purity polyphenols from walnuts and determine polyphenols antioxidant activities was extracted. Using the macroporous resin leaching and adsorption to extract polyphenols crudely, the rough extraction of green peel polyphenols was purified further by adjusting pH, centrifuge precipitation and cooling separation crystals. The green peel polyphenols in crude extract and purified solution were analyzed comparatively by high performance liquid chromatography(HPLC). The results showed that the total content of purified polyphenols exceeded 80%. The effects of elution flow rate on the adsorption performance of macroporous resin and pH on centrifugal precipitation process were discussed. The antioxidant properties of green peel polyphenols were analyzed mainly.