弥漫性大B细胞淋巴瘤组织CMYC、叉头框转录蛋白1、ki-67蛋白表达及临床意义

Expression and Clinical Significance of CMYC,FOXP1 and ki-67 in Diffuse Large B Cell Lymphoma

目的:探讨弥漫性大B细胞淋巴瘤(DLBCL)组织CMYC、叉头框转录蛋白1(FOXP1)、ki-67蛋白表达及临床意义。方法:选择2013年2月~2018年1月我院收治的DLBCL患者100例,以免疫组织化学法分别检测DLBCL组织及癌旁组织中CMYC、FOXP1、ki-67蛋白表达情况,并分析DLBCL组织中上述蛋白表达和患者临床病理特征的关系。此外,对所有患者均进行为期2年的随访观察,将其按照生存情况分成存活组69例和死亡组31例,比较两组CMYC、FOXP1、ki-67蛋白表达情况。结果:DLBCL组织CMYC、FOXP1、ki-67蛋白阳性表达率均高于癌旁组织(P<0.05)。国际预后指数(IPI)评分为3~4分的患者DLBCL组织CMYC蛋白阳性表达率低于IPI评分为0~2分的患者(P<0.05)。病理分型为生发中心B细胞来源(GCB)、IPI评分为3~4分的患者DLBCL组织FOXP1蛋白阳性表达率分别低于病理分型为非生发中心B细胞来源(non-GCB)、IPI评分为0~2分的患者(P<0.05)。年龄<60岁、Ann Arbor分期为Ⅰ~Ⅱ期、IPI评分为3~4分的患者DLBCL组织ki-67蛋白阳性表达率分别低于年龄≥60岁、Ann Arbor分期为Ⅲ期、IPI评分为0~2分的患者(P<0.05)。死亡组CMYC、FOXP1、ki-67蛋白阳性表达率均高于存活组(P<0.05)。结论:DLBCL组织CMYC、FOXP1、ki-67蛋白表达均与IPI评分关系密切,FOXP1蛋白表达与病理分型有关,而ki-67蛋白表达与年龄以及Ann Arbor分期有关,DLBCL患者不良预后可能与上述三项蛋白阳性表达率的异常升高有关。

Objective:To investigate the expression and clinical significance of CMYC,forkhead frame transcription protein 1(FOXP1)and ki-67 proteins in diffuse large B-cell lymphoma(DLBCL).Methods:100 patients with DLBCL who were admitted to our hospital from February 2013 to January 2018 were included in the study.Immunohistochemistry was used to detect the expression of CMYC,FOXP1 and ki-67 proteins in DLBCL tissues and pericarcinomatous tissues.The relationship between the clinicopathological characteristics and the protein expression in DLBCL tissues of the patients were analyzed.In addition,all patients were followed for 2 years,and they were divided into survival group of 69 cases and death group of 31 cases according to their survival conditions.The expressions of CMYC,FOXP1 and ki-67 proteins in the two groups were compared.Results:The positive expression rates of CMYC,FOXP1 and Ki-67 in DLBCL tissues were higher than those in pericarcinomatous tissues(P<0.05).The positive expression rate of CMYC protein in DLBCL tissues of patients with International Prognostic Index(IPI)score of 3-4 points was lower than that of patients with IPI score of 0-2 points(P<0.05).The positive expression rate of FOXP1 protein in DLBCL tissues of patients with pathological classification of Germinal center B cell source(GCB)and IPI score of 3-4 points were lower than those of patients with pathological classification of non-Germinal center B cell source(non-GCB)and IPI score of 0-2 points(P<0.05).The positive expression rate of ki-67 protein in DLBCL tissues of patients with Age<60 years old,Ann Arbor stage wasⅠ~Ⅱ,IPI score for 3~4 points were respectively lower than those of patients with age≥60 years old,Ann Arbor stage wasⅢ,IPI score for 0~2 points(P<0.05).The positive expression rates of CMYC,FOXP1 and ki-67 protein in the death group were higher than those in the survival group(P<0.05).Conclusion:The CMYC,FOXP1 and ki-67 rotein expression in DLBCL tissues are closely related to IPI score,and FOXP1 is related to pathological typing,while ki-67 rotein expression is related to age and Ann Arbor stage.With the positive expression rates abnormal increase of the above three proteins,the prognosis of patients with DLBCL are worse.