三阴性乳腺癌组织AR、MMP-9、E-cadhenrin表达与临床病理特征及预后的关系研究

Study on the Relationship between Expression of AR,MMP-9,E-cadhenrin and Clinicopathological Characteristics and Prognosis of Triple Negative Breast Cancer Tissue

目的:探讨三阴性乳腺癌(TNBC)组织雄激素受体(AR)、基质金属蛋白酶9(MMP-9)、E-钙黏蛋白(E-cadhenrin)表达与临床病理特征及预后的关系。方法:选取2014年1月至2017年1月徐州医科大学附属沭阳医院收集的91例TNBC患者手术切除的癌组织和癌旁组织以及61例乳腺增生组织(对照组)石蜡标本,免疫组化法检测AR、MMP-9、E-cadhenrin表达情况。分析AR、MMP-9、E-cadhenrin表达与TNBC患者临床病理参数之间的关系,Kaplan-Meier生存曲线、COX风险比例回归分析AR、MMP-9、E-cadhenrin表达与TNBC患者预后的关系。结果:TNBC癌组织中MMP-9阳性表达率高于癌旁组织和对照组,AR、E-cadhenrin阳性表达率低于癌旁组织和对照组(P<0.05)。AR表达与分化程度、组织学分级、淋巴结状态有关,MMP-9表达与组织学分级、淋巴结状态、Ki-67表达有关,E-cadhenrin表达与组织学分级、淋巴结状态有关(P<0.05)。Kaplan-Meier生存分析结果显示MMP-9阳性表达患者生存率均低于MMP-9阴性表达患者,AR、E-cadhenrin阴性表达患者生存率均低于AR、E-cadhenrin阳性表达患者(P<0.05)。COX风险比例回归分析结果显示淋巴结状态N1-2、MMP-9阳性表达、AR、E-cadhenrin阴性表达是TNBC患者预后不良的危险因素(P<0.05)。结论:MMP-9过度表达和AR、E-cadhenrin表达缺乏与TNBC患者肿瘤恶性侵袭行为和预后不良有关,评价AR、MMP-9、E-cadhenrin表达状态可为TNBC患者预后预测提供一定的依据。

Objective:To investigate the relationship of expression of androgen receptor(AR),matrix metalloproteinase 9(MMP-9),E-cadhenrin with clinicopathological characteristics and prognosis of triple negative breast cancer(TNBC)tissue.Methods:Cancerous tissue and paracancerous tissue which were resected surgically in 91 patients with TNBC and 61 cases of breast hyperplasia(control group)paraffin specimens which were collected in Shuyang Hospital Affiliated to Xuzhou Medical University from January2014 to January 2017 were selected,expression of AR,MMP-9,E-cadhenrin was measured by immunohistochemical method.The relationship between the expression of AR,MMP-9,E-cadhenrin and the clinicopathological parameters of TNBC was analyzed,the relationship between the expression of AR,MMP-9,E-cadhenrin and the prognosis of patients with TNBC was analyzed by Kaplan Meier survival curve,Cox risk ratio regression.Results:MMP-9 positive expression rate in TNBC cancerous tissue were higher than paracancerous tissue tissues and control group,AR,E-cadhenrin positive expression rate were lower than paracancerous tissues and control group(P<0.05).Expression of AR was correlated with degree of differentiation,histological grade,lymph node status,expression of MMP-9 was correlated with histological grade,lymph node status,Ki-67 expression,and expression of E-cadhenrin was correlated with histological grade,lymph node status(P<0.05).Kaplan-Meier survival analysis showed that the survival rate of patients with positive expression of MMP-9 was lower than those of patients with negative expression of MMP-9,and the survival rate of patients with negative expression of AR,E-cadhenrin was lower than that of patients with positive expression of AR,E-cadhenrin(P<0.05).COX regression analysis showed that lymph node status N1-2,positive expression of MMP-9,AR,E-cadhenrin negative expression were risk factors for poor prognosis of TNBC patients(P<0.05).Conclusion:Excessive expression of MMP-9,lack of AR,E-cadhenrin expression were associated with malignant invasion behavior of tumor and poor prognosis in TNBC patients,and evaluation of expression of AR,MMP-9,E-cadhenrin could provide certain basis for prognosis prediction in TNBC patients.