EGFR、GPER在子宫内膜异位症组织中表达及其相关性

Expressions of epidermal growth factor receptor and G protein coupled estrogen receptor in endometrial tissue of the women with endometriosis and their correlation

目的:检测表皮生长因子受体(EGFR)和G蛋白偶联雌激素受体(GPER)在子宫内膜异位症(EMT)组织中表达及临床意义。方法:选择2018年1月-2020年1月在本院就诊并手术治疗的EMT患者42例作为观察组,另选同期因子宫平滑肌瘤而行全子宫切除手术患者31例作为对照组。采用免疫组织化学染色检测内膜组织中EGFR、GPER蛋白表达情况,采用qRT-PCR法检测EMT组织与正常子宫内膜组织中EGFR和GPER mRNA表达水平,分析EMT组织中EGFR与GPER mRNA表达相关性。结果:EMT异位与在位内膜组织中EGFR、GPER mRNA及蛋白表达水平无差异但均高于正常子宫内膜组织(P<0.05);EMT在位内膜组织中EGFR、GPER蛋白在增生期阳性表达率表达高于分泌期,EMT异位内膜组织中EGFR、GPER蛋白在Ⅲ-Ⅳ期阳性表达率高于Ⅰ~Ⅱ期(均P<0.05);Pearson分析结果显示,EMT组织中EGFR与GPER mRNA表达水平呈正相关(r=0.531,P=0.000)。结论:EMT组织中EGFR、GPER高表达,与EMT的发生及临床分期可能相关,二者在EMT的发生发展中可能存在相互协同作用。

Objective:To detect the expressions of epidermal growth factor receptor(EGFR)and G protein coupled estrogen receptor(GPER)in endometrial tissue of the women with endometriosis(EMT),and to explore the relationship between EGFR level and GPER level and their clinical significance.Methods:From Jan 2018 to Jan 2020,42 patients with EMT were selected in observation group,and 31 patients with hysterectomy because of leiomyoma of uterus were selected in control group.The expression of protein of EGFR and GPER in endometrial tissue was detected by immunohistochemistry,and the expression levels of EGFR and GPER mRNA in the tissue of EMT and normal endometrium were detected by qRT PCR.The correlation between EGFR mRNA expression and GPER mRNA expression in endometrial tissue of the women with EMT was analyzed.Results:The expression levels of EGFR and GPER mRNA and protein in ectopic and eutopic endometrium of EMT were significant higher than those in normal endometrium(P<0.05),while there were no significant difference in the expression levels of EGFR,GPER mRNA and protein between ectopic and eutopic endometrium of EMT(P>0.05).In eutopic endometrium of EMT,the protein positive expression rates of EGFR and GPER in proliferative phase endometrium was significant higher than that in secretory phase endometrium(P<0.05).In ectopic endometrium of the women with EMT,the positive expression rate of EGFR and GPER in endometrium of the women with stage Ⅲ-Ⅳ of EMT was significant higher than that of the women with stage Ⅰ-Ⅱ of EMT(P<0.05).Pearson analysis showed that the expression level of EGFR mRNA were positively correlated with the expression level of GPER mRNA(r=0.531,P=0.000).Conclusion:The expresses of EGFR and GPER in endometrial tissue of the women with EMT are higher,which may play a synergistic role in the occurrence and clinical stage of EMT.