摘要
目的探究内向整流钾通道Kir4.1在恶性胶质瘤中的表达情况,通过数据库并结合文献分析其与胶质瘤癫痫、水肿等症状的相关性及其相关机制。方法利用Oncomine数据库分析TCGA数据集中胶质母细胞瘤与正常脑组织相比的表达情况,收集临床不同级别胶质瘤患者的标本及病理信息,分析不同级别胶质瘤中Kir4.1的表达情况。利用cBioPortal数据库分析胶质瘤中Kir4.1突变情况。结果 Oncomine数据库因数据集样本量差异而显示出较大的结果差异性,RTPCR和免疫组化显示高级别胶质瘤比低级别胶质瘤的Kir4.1表达量更少,但是低级别胶质瘤的Kir4.1表达量比正常脑组织表达量高,差异具有统计学意义(P<0.05)。cBioPortal数据库显示Kir4.1突变概率在胶质母细胞瘤中极低,部分基因组改变与突变数量之间差异均有统计学意义(P<0.05)。MSH2、ERRFI1、CTNNB1、SEPRINE1、FOXM1、RAD50等基因在改变组中高表达,INPP4B、XBP1、AXL等基因在非改变组里高表达。结论 Kir4.1在不同级别的胶质瘤中表达具有差异性,高级别的胶质瘤具有更低的Kir4.1的表达量,且Kir4.1的表达可能和高级别胶质瘤患者发生癫痫、水肿等临床症状相关,为未来探索Kir4.1在胶质母细胞瘤中的潜在机制提供了新的思路。
Objective To explore the expression of inward rectifier potassium channel Kir4.1 in malignant gliomas and its correlation and correlation mechanism with epilepsy,edema and other symptoms of gliomas through database and document analysis.Methods Oncomine database was used to analyze the expression of glioblastoma compared with normal brain tissue in TCGA data set.Specimens and pathological information of patients with different clinical grades of glioma were collected to analyze the expression of Kir4.1 in different grades of glioma.Kir4.1 mutations in gliomas were analyzed by cbioportal database.Results The results of oncomine database showed great differences due to the differences of sample size.RT-PCR and immunohistochemistry showed that the expression of Kir4.1 in high-grade glioma was less than in low-grade glioma,but the expression of Kir4.1 in low-grade glioma was higher than in normal brain tissues,and the difference was statistically significant(P<0.05).The cbioportal database showed that the mutation probability of Kir4.1 was extremely low in glioblastoma,and the difference between partial genome alteration and the number of mutations was statistically significant(P<0.05).MSH2,ERRFI1,CTNNB1,SEPRINE1,FOXM1,RAD50 and other genes were highly expressed in the altered group,while INPP4B,XBP1,AXL and other genes were highly expressed in the non-altered group.Conclusion The expression of Kir4.1 is different in different grades of glioma. High grade glioma has lower expression of Kir4.1, and the expression of Kir4.1 may be related to epilepsy, edema and other clinical symptoms in patients with high grade glioma, which provides a new idea for exploring the potential mechanism of Kir4.1 in glioblastoma in the future.
作者
张婵
薛强
田锐锋
陈晓燕
Zhang Chan;Xue Qiang;Tian Ruifeng;Chen Xiaoyan(Department of Outpatient,The First Affiliated Hospital of Air Force Medical University,Xi’an 710032,China;Department of Cardiology,The First Affiliated Hospital of Air Force Medical University,Xi’an 710032,China;Department of Infection,The First Affiliated Hospital of Air Force Medical University,Xi’an 710032,China;Department of Neurosurgery,The First Affiliated Hospital of Air Force Medical University,Xi’an 710032,China)
出处
《中华神经创伤外科电子杂志》
2021年第4期224-234,共11页
Chinese Journal Of Neurotraumatic Surgery：Electronic Edition
