幽门螺杆菌无创性分子生物学检测方法研究进展

Research progress of non-invasive molecular biological methods for detecting Helicobacter pylori

幽门螺杆菌与慢性活动性胃炎、消化性溃疡、胃黏膜相关淋巴组织淋巴瘤和胃癌等消化系统疾病密切相关,是目前唯一被世界卫生组织列为Ⅰ类致癌原的病原菌。随着分子生物学技术的发展,关于幽门螺杆菌鉴定、耐药或毒力基因分析的分子生物学方法不断更新,对幽门螺杆菌的精准诊疗具有重要意义。从口腔和粪便样本中直接检测幽门螺杆菌的无创性分子生物学检测方法具有简便、高效、经济和非侵入性等优点,已成为相关领域的研究热点。文章介绍了幽门螺杆菌无创性分子生物学检测方法的研究进展,并对多种幽门螺杆菌常规检测方法的利弊进行比较,以期为幽门螺杆菌的临床诊治提供参考。

Helicobacter pylori is closely related to the occurrence of digestive system diseases,such as chronic active gastritis,peptic ulcer,gastric mucosa-associated lymphoid tissue(MALT)lymphoma and gastric cancer.It is currently the only pathogen that is classified as classⅠcarcinogen by World Health Organization.With the development of molecular biological technology,a variety of molecular biological methods for Helicobacter pylori identification,drug resistance and virulence analysis have emerged,which are of significance to the accurate diagnosis and treatment of Helicobacter pylori infection.The molecular biological methods of non-invasive detection of Helicobacter pylori from oral cavity or fecal samples directly have the advantages of simplicity,efficiency,economy and non-invasiveness,and have become a research hot-spot recently.This review will analyze and compare the pros and cons of various conventional methods for detecting Helicobacter pylori,focusing on the research progress of non-invasive molecular biological methods for detecting Helicobacter pylori.