摘要
目的分析2014—2019年环鄱阳湖地区H9N9亚型禽流感病毒的分子特征。方法2014—2019年采集环鄱阳湖地区禽类相关环境标本31854份,运送至国家流感中心(CNIC)并进行无特定病原体(SPF)鸡胚法病毒分离。经甲型流感病毒快速诊断试剂盒进行流感病毒阳性筛查,收获所有甲型流感病毒(IAV)阳性鸡胚尿囊液。病毒分离阳性毒株进行基因深度测序,使用CLC、CD-HIT、MAFFT 7.475、MEGA 6.06、FastTree、FigTree v1.4.4软件进行序列分析。结果2014—2019年环鄱阳湖地区共检测到18株H9N9亚型禽流感病毒(AIVs)。对病毒全基因组序列做系统进化分析,结果表明均由H9N2亚型AIVs和H7N9亚型AIVs基因重配产生。江西省禽类相关环境标本中分离的H9N9亚型AIVs的NA基因与中国东部省份分离的H9N9亚型AIVs的NA基因处于不同分支。氨基酸位点分析显示这些H9亚型AIVs均为低致病性禽流感病毒,但HA基因上的Q226L,PA蛋白550L位点、M1蛋白的N30D和T215A位点以及NS1蛋白的P189D、F103L和M106I等关键位点发生替代。结论2014—2019年我国环鄱阳湖地区的H9N9亚型AIVs系H9N2和H7N9亚型流感病毒重配而来,推测其出现与H9N2、H7N9在2013—2017年期间同时高发流行密切相关。氨基酸位点分析提示该病毒具有感染人的潜力。尽管目前H9N9亚型AIVs未造成人感染病例,仍要加强不同亚型AIVs之间重配病毒的监测和研究。
Objective To understand the molecular characteristics of avian influenza A(H9N9)virus isolated in Poyang Lake area in Jiangxi province between 2014 and 2019.Methods A total of 31854 poultry related environmental samples were collected in Poyang Lake area between 2014 and 2019.The samples were transported to Chinese National Influenza Center(CNIC)at low temperatures for virus isolation.All allantoic fluids were tested by influenza A virus fast diagnostic kits and influenza A virus positive allantoic fluids were harvested,then,the isolates were deeply sequenced.Software CLC,CDHIT,MAFFT 7.475,MEGA 6.06,FastTree and FigTree v1.4.4 were used for the sequence analysis.Results Between 2014 and 2019,a total of 18 influenza A(H9N9)virus strains were isolated in Poyang Lake area.Phylogenetic analysis of their full genome showed that they were reassortant of H9N2 virus and H7N9 virus.The NA segments of the H9N9 virus isolated in Jiangxi and the H9N9 virus isolated from Eastern China were in different sub-clade.Amino acid sites analysis of these H9 subtype viruses showed that they belonged to low pathogenic avian influenza virus,but some crucial amino acids substitution had been found,such as Q226L in HA protein,550L in PA protein,N30D,T215A in M1 protein and P189D,F103L and M106I in NS1 genes.Conclusion The influenza A(H9N9)virus strains isolated in our study during 2014–2019 were the reassortant of H9N2 and H7N9 viruses,which might be highly associated with the high incidences of H9N2 virus and H7N9 virus infections during 2013–2017.Although,there were no reported about human infection with influenza A(H9N9)virus,the genetic characteristic analysis results has shown it has potential to infect human.It is necessary to strengthen the continuous surveillance and research on reassortant of avian influenza viruses.
作者
曾晓旭
焦铭
陈涛
谢昀
宋文涛
刘佳
张恒
杨磊
傅伟杰
陈海婴
王大燕
Zeng Xiaoxu;Jiao Ming;Chen Tao;Xie Yun;Song Wentao;Liu Jia;Zhang Heng;Yang Lei;Fu Weijie;Chen Haiying;Wang Dayan(Chinese National Influenza Center,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Jiangxi Center for Disease Control and Prevention,Nanchang 330029,Jiangxi,China;Nanchang Center for Disease Control and Prevention,Nanchang 330038,Jiangxi,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2021年第7期659-665,共7页
Disease Surveillance
基金
国家科技重大专项(No.2020ZX10001–016–004,No.2018ZX10711–001)。
关键词
禽流感病毒
H9N9亚型
进化
重配
Avian influenza virus
H9N9 subtype
Evolution
Reassortant