摘要
目的建立我国分离库蚊黄病毒(Culex flavivirus,CxFV)毒株(SDDM06-11)的病毒滴度测定方法。方法在6孔细胞培养板中培养C6/36细胞,感染库蚊黄病毒,加入甲基纤维素培养4 d,结晶紫进行染色,通过统计空斑数量计算病毒的噬斑形成单位。结果使用甲基纤维素-结晶紫空斑方法观察,发现CxFV(SDDM06-11)毒株感染C6/36细胞后形成清晰的圆形噬斑,大小如针尖,成功测定CxFV(SDDM06-11株)滴度在106.85 pfu/ml。结论甲基纤维素-结晶紫空斑法可用于测定CxFV滴度。
Objective To establish a method for determining the virus titer of the Culex flavivirus(CxFV)strain(SDDM06-11)isolated in China.Methods C6/36 cells were cultured in a 6-well cell culture plate and were then inoculated with CxFV.Methylcellulose was added to the cells and cultured for 4 days.Crystal violet was used for staining,and the number of plaques was determined to calculate the plaque forming unit of the virus.Results Observation of plaques using methylcellulose and crystal violet showed that CxFV(SDDM06-11)strain formed clear round plaques with the size of a needle tip after infecting C6/36 cells,and the virus titer of SDDM06-11 strain was successfully determined as 106.85 pfu/ml.Conclusion The methylcellulose-crystal violet plaque forming assay can be used to determine the titer of CxFV.
作者
何英
殷启凯
付士红
王环宇
HE Ying;YIN Qi-kai;FU Shi-hong;WANG Huan-yu(Department of Arboviruses,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《中国媒介生物学及控制杂志》
CAS
北大核心
2021年第4期412-414,共3页
Chinese Journal of Vector Biology and Control
基金
国家科技重大专项(2018ZX10734-401-001,2018ZX10711001)
传染病预防控制国家重点实验室课题(2015SKLID505)。
