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大熊猫CD4分子流式细胞术检测用抗体的初步研究

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摘要 流式细胞术是一种利用流式细胞仪快速定量分析细胞群的物理化学特性以及根据这些理化特性精确分选细胞的技术。本研究利用流式细胞仪对北京动物园圈养大熊猫外周血液内白细胞CD4蛋白进行检测,成功筛选出适用于大熊猫外周血CD4蛋白检测的抗体,为研究大熊猫的免疫学特征及其遗传机制提供新的科研思路及理论依据。
出处 《上海畜牧兽医通讯》 2021年第4期7-10,共4页 Shanghai Journal of Animal Husbandry and Veterinary Medicine
基金 国家重点研发计划(2017YFD0501701)大熊猫与虎遗传谱系与繁殖技术研究。
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  • 1赵德明,张仲秋,沈建忠.猪病学[M].第9版.北京:中国农业大学出版社,2008:853-854.
  • 2Floegel-Niesmann G,Bunzenthal C,Fischer S,et al.Virulence of recent and former classical swine fever virus isolates evaluated by their clinical and pathological signs[J].J Vet Med B Infect Dis Vet Public Health,2003,50:214-220.
  • 3Ganges L,Barrera M,Nunez J I,et al.A DNA vaccine expressing the E2 protein of classical swine fever virus elicits T cell responses that can prime for rapid antibody production and confer total protection upon viral challenge[J].Vaccine,2005,23,3741-3752.
  • 4Gomez-Villamandos J C,Carrasco L,Bautista M J,et al.African swine fever and classical swine fever: a review of the pathogenesis[J].Dtsch Tierarztl Wochenschr,2003,110:165-169.
  • 5Lee W C,Wang C S,Chien M S.Virus antigen expression and alterations in peripheral blood mononuclear cell subpopulations after classical swine fever virus infection[J].Vet Microbiol,1999,67:17-29.
  • 6Moennig V,Floegel-Niesmann G,Greiser-Wilke I.Clinical signs and epidemiology of classical swine fever: a review of new knowledge[J].Vet J,2003,165:11-20.
  • 7Saalmuller A,Reddehase M J,Buhring H J,et al.Simultaneous expression of CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes[J].Eur J Immunol,1987,17:1297-1301.
  • 8Shimizu M,Yamada S,Nishimori T.Cytocidal infection of hog cholera virus in porcine bone marrow stroma cell cultures[J].Vet Microbiol,1995,47:395-400.
  • 9Vandeputte J,Too H L,Ng F K,et al.Adsorption of colostral antibodies against classical swine fever,persistence of maternal antibodies,and effect on response to vaccination in baby pigs[J].Am J Vet Res,2001,62:1805-1811.
  • 10金伯泉.医学免疫学[M].北京:人民卫生出版社,2011.

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