DNA微阵列芯片在皮肤分枝杆菌感染中的早期诊断价值

Diagnostic value of DNA microarray chip for cutaneous mycobacterial infections

目的研究DNA微阵列芯片在皮肤分枝杆菌感染早期诊断中的临床应用价值。方法应用传统方法(包括病理学以及培养和测序鉴定)和DNA微阵列芯片技术对6例临床疑似皮肤分枝杆菌感染患者的皮肤组织进行检测。结果6例患者的发病诱因多有海鲜接触史或外伤史,表现为单发或是呈淋巴管样排列模式;传统的细菌培养有5例患者阳性,经过测序鉴定分别为海分枝杆菌4例、龟分枝杆菌1例;DNA微阵列芯片技术检测6例患者均为阳性,其中海分枝杆菌5例,龟分枝杆菌1例;DNA微阵列芯片技术阳性率(6/6)高于传统的培养技术(5/6),此外检测时间也远低于传统培养技术。结论DNA微阵列芯片技术具有简便、快速、高敏感等特点,可鉴定皮肤分枝杆菌感染的致病菌种,为临床做出早期诊断和治疗提供依据。

Objective To study the diagnostic value of DNA microarray chip for cutaneous mycobacterial infections.Methods Six skin tissues from clinical cutaneous mycobacterial infections suspected cases were underwent pathogenic detection by traditional bacteria culture method and DNA microarray chip technology.Results All patients had history of aquatic exposure or trauma,the clinical manifestation patterns were single lesion or sporotrichoid infection type.Among 6 suspected cases,there were 5 cases with positive culture of mycobacteria strains,which include 4 strains of Mycobacterium marinum,1 strain of M.chelonae.The chip examinations were positive in 6 cases,included 5 M.marinum cases and 1 M.chelonae case.Compared with traditional bacteria culture method,DNA microarray chip technology had a higher sensitivity(6/6 vs 5/6)and was less time-consuming.Conclusion The DNA microarray ship technology provided a simple,rapid,and highly sensitive clinical assay,it could be used for mycobacteria species identification of skin tissue samples from cutaneous mycobacterial infections,allowing earlier aetiological diagnosis and pathogen-adapted antimicrobial therapy.