ZBTB40基因在人类细胞系和肝癌组织及小鼠各组织中的表达研究

Study on the Expression of ZBTB40 Gene in Human Cell Lines,Hepatocellular Carcinoma and Mouse Tissues

ZBTB40(zinc finger and BTB domain containing 40)是ZBTB转录因子家族中的成员,其在高等动物中的组织表达情况和分子功能尚不清楚。为初步探讨ZBTB40基因的表达和生理功能,本研究系统检测了其在8种人类细胞系和9种小鼠组织中的mRNA及蛋白质相对丰度。结果显示,ZBTB40在大多数(6/8)人类细胞系和3种小鼠组织(肝脏、胸骨及睾丸)中有表达,且在小鼠肝脏中的丰度显著高于其他组织,提示其与肝脏生理功能相关。为验证此假设,进一步检测了ZBTB40在5对人肝细胞癌及癌旁组织中的表达水平。结果显示,ZBTB40在肝癌细胞的细胞核中高表达,在癌基质中表达量很低。生存分析显示,ZBTB40蛋白的表达水平与肝癌病人的生存时间显著负相关,提示其可能为肝癌潜在的预后生物标志物。此外,本研究还构建了ZBTB40基因慢病毒表达质粒,将其感染U2OS细胞后获得了人ZBTB40稳定表达的细胞株,为后续分子机制及功能研究打下了基础。

Zinc finger and BTB domain containing 40(ZBTB40)is a member of ZBTB transcription factor family.The tissue expression pattern and molecular functions of ZBTB40 in mammal are still unclear.In or-der to investigate the expression pattern and physiological function of the ZBTB40 gene,the relative abun-dance of ZBTB40 mRNA and protein in eight human cell lines and nine mouse tissues was detected.The re-sults showed that ZBTB40 expressed in most of the human cell lines(6/8)and three kinds of mouse tissues(liver,sternum and testis).The ZBTB40 abundance in mouse liver was significantly higher than that in other tis-sues,suggesting that ZBTB40 may be important for physiological functions of liver.To validate the hypothe-sis,the level of ZBTB40 in five pairs of human hepatocellular carcinoma(HCC)and adjacent tissues was exa-mined.The results showed that ZBTB40 was highly expressed in the nucleus of liver cancer cells,but the expression in carcinoma stromal cells was the lowest.Survival analysis suggested that the expression of ZBTB40 was negatively associated with the overall survival time of HCC patients,indicating that it may be a potential prognostic biomarker.In addition,the viral expression plasmid of ZBTB40 gene was constructed,and cell lines stably expressing human ZBTB40 were obtained after U2OS cells were infected,which laid a foundation for further molecular mechanism and functional research.