摘要
通过在体外构建模拟消化和发酵体系,研究牦牛骨胶原蛋白肽对小鼠粪便菌群体外厌氧发酵的影响。发酵过程中取样监测pH,乳酸和短链脂肪酸的含量变化。发酵结束后,采用16S rRNA高通量测序技术测定小鼠粪便菌群的组成。结果表明,添加牦牛骨胶原蛋白肽后,发酵液中短链脂肪酸的含量显著增多(P<0.01)。与未添加牦牛骨胶原蛋白肽的对照组相比,高剂量组的乙酸和丙酸含量分别增加86.7%,83.7%。此外,微生物多样性发生改变,粪便菌群组成也发生了显著变化。低剂量和高剂量组与对照组相比,在门水平上,放线菌门相对丰度分别上升69.3%和52%,在属水平上,双歧杆菌的相对丰度分别提高70.3%和52.4%。粪便菌群功能预测显示,小鼠粪便菌群的碳水化合物及氨基酸代谢功能有一定的提高。因此,牦牛骨胶原蛋白肽对小鼠肠道菌群可能具有一定的调节作用。
The effects of yak bone collagen peptides(YBCP)on the fermentation of mice fecal microbiota were studied by constructing a simulated digestion and fermentation system in vitro.The pH,short-chain fatty acids(SCFA)and lactic acid were detected during the fermentation.At the end of the trial,the composition of gut microbiota was analyzed by 16S rRNA sequencing.The results showed that the concentration of SCFA and lactic acid in the YBCP added groups were significantly increased when compared with that in the control group(P<0.01).The concentration of acetic acid and propionic acid in the high-dose group was increased by 86.7%and 83.7%,respectively.Moreover,species diversity and gut microbiota composition were altered.Compared with the control group,the relative abundance of Actinobacteria in the low-dose and high-dose groups was increased by 69.3%and 52%,respectively.At the genus level,the relative abundance of Bifidobacterium in the low-dose and high-dose groups was elevated by 70.3%and 52.4%,respectively.The predicted functions of the fecal microbiota indicated that the carbohydrate and amino acid metabolism of fecal microbiota in mice had a certain improvement in the YBCP groups.These results indicated that the YBCP might have the ability to modulate the structure of gut microbiota in mice.
作者
刘春雨
衣大龙
杨玉亮
辛瑜
顾正华
刘怀高
郭自涛
张梁
LIU Chunyu;YI Dalong;YANG Yuliang;XIN Yu;GU Zhenghua;LIU Huaigao;GUO Zitao;ZHANG Liang(National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China;Anhui Guotai Biotechnology Co.Ltd.,Xuancheng 242100,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第16期59-65,共7页
Food and Fermentation Industries
基金
轻工业技术与工程国家双一流学科(LITE2018-22)。
关键词
牦牛骨胶原蛋白肽
肠道菌群
模拟消化与发酵
短链脂肪酸
代谢功能
yak bone collagen peptides
gut microbiota
simulated digestion and fermentation
short-chain fatty acids
metabolic function