蒙药那如-3味的醇提工艺优化

Optimization of Ethanol Extraction Technology of Mongolian Medicine Naru-3

目的:优化蒙药那如-3味的醇提工艺。方法:以苯甲酰乌头原碱、苯甲酰新乌头原碱、苯甲酰次乌头原碱、乌头碱、新乌头碱、次乌头碱、胡椒碱和没食子酸含量的综合评分为考察指标,料液比、乙醇体积分数、提取时间为考察因素,采用L9(34)正交实验设计优化蒙药那如-3味的醇提工艺。结果:最优醇提工艺为料液比1∶10(g/mL)、乙醇体积分数75%、提取时间1.5 h。经3次实验验证,那如-3味醇提物中上述8种成分的平均含量分别为1.69、1.48、14.69、0.28、0.05、0.08、26.01、17.33 mg/g(RSD为0-4.96%,n=3),平均综合评分为19.03分(RSD=1.42%,n=3)。结论:优化所得那如-3味醇提工艺稳定、可行。

OBJECTIVE:To optimize ethanol extraction technology of Mongolian medicine Naru-3.METHODS:The L9(34)orthogonal design was used to optimize ethanol extraction technology of Mongolian medicine Naru-3 with solid-liquid ratio,ethanol volume fraction and extraction time as factors,using comprehensive scores for the contents of benzoylaconitine,benzoylneoaconitine,benzoylhypoaconitine,aconitine,neoaconitine,hypoaconitine,piperine and gallic acid as indexes.RESULTS:The optimal ethanol extraction technology was that solid-liquid ratio of 1∶10(g/mL),ethanol volume fraction of 75%,extracting for 1.5 h.After 3 times of validation tests,average contents of above 8 components in ethanol extract from Naru-3 were 1.69,1.48,14.69,0.28,0.05,0.08,26.01,17.33 mg/g(RSDs were 0-4.96%,n=3),respectively.Average comprehensive score was 19.03(RSD=1.42%,n=3).CONCLUSIONS:The optimal ethanol extraction technology of Mongolian medicine Naru-3 is stable and feasible.