陕西省布鲁氏菌菌株多位点序列分析研究

Multi-locus sequence typing of Brucella strains in Shaanxi Province

目的对陕西省分离的77株布鲁氏菌菌株进行遗传多态性分析,了解其遗传特征及进化关系。方法应用多位点序列分型(Multiple locus sequence typing,MLST)技术,测定77株陕西省地区布鲁氏菌分离株的7个管家基因、1个外膜蛋白基因及1个基因间区的序列,确定各菌株序列型(STs),运用BioNumerics(Version 7.6)构建最小进化树,分析菌株间遗传进化关系。结果74株分离株(47株羊种3型、10株羊种1型、7株羊种2型、10株羊种变异型)序列型为ST8型,2株分离株(牛种生物型)序列型为ST2,1株分离株(猪种1型)序列型为ST14型。结论不同生物型布鲁氏菌的MLST分型结果稳定,羊种3型(ST8型)布鲁氏菌是陕西省主要流行菌株;MLST分型作为传统生物分型的技术补充,建立了陕西省布鲁氏菌基因分型数据库,对本省今后人间布病的防控具有科学指导意义。

Genetic polymorphisms of 77 Brucella strains isolated from Shaanxi Province were analyzed to assess their genetic characteristics and evolutionary relationships.Multiple locus sequence typing(MLST)was used to identify the sequence types(STs),on the basis of seven housekeeping genes,one outer membrane protein gene and one intergene region,in 77 Brucella isolates from Shaanxi Province.A minimum evolutionary tree was constructed to analyze the genetic and evolutionary relationships among the strains in BioNumerics(Version 7.6).The results showed that 74 strains(47 Brucella melitensis biovar 3,ten Brucella melitensis biovar 1,seven Brucella melitensis biovar 2,and ten Brucella melitensis biovar variant)were ST8,two strains(Brucella abortus)were ST2,and one strain(Brucella suis biovar 1)was ST14.The MLST results for the different biological types of Brucella were stable,and Brucella Melitensis biovar 3(ST8)was found to be the main epidemic strain in Shaanxi Province.MLST genotyping as a technical supplement to traditional biological typing was used to establish a Brucella genotyping database,thus providing scientific guidance for the prevention and control of human brucellosis in Shaanxi Province in the future.